The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 103
each slide with phase contrast microscopy . Examine slides for good cell
concentration , chromosome spreading , morphology , and mitotic index . Good
chromosome preparations are spread adequately for easy counting and analysis
.
each slide with phase contrast microscopy . Examine slides for good cell
concentration , chromosome spreading , morphology , and mitotic index . Good
chromosome preparations are spread adequately for easy counting and analysis
.
Pagina 294
Store slides in trays used for cleaning ( 20 - slide Wheaton staining dish , Thomas
# 8541 - H10 ) at 4 ° C in distilled H2O . 3. Remove a slide from the tray , and
hold it lengthwise at a 45 ° angle so that most of the H2O drains off of the slide . 4.
Store slides in trays used for cleaning ( 20 - slide Wheaton staining dish , Thomas
# 8541 - H10 ) at 4 ° C in distilled H2O . 3. Remove a slide from the tray , and
hold it lengthwise at a 45 ° angle so that most of the H2O drains off of the slide . 4.
Pagina 433
Some laboratories prefer to scan slides using Q - banding stains ; this method
eliminates the initial step of staining slides with Giemsa and photographing cells
and is thus more efficient . For complex rearrangements , it may be necessary to ...
Some laboratories prefer to scan slides using Q - banding stains ; this method
eliminates the initial step of staining slides with Giemsa and photographing cells
and is thus more efficient . For complex rearrangements , it may be necessary to ...
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |