The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 49
Pagina 48
... spreading , whereas heavier applications will in- crease spreading . The amount of methanol in the fix- flood can be increased to provide faster evaporation , which may be helpful when chromosomes tend to scatter . Dry slides are also ...
... spreading , whereas heavier applications will in- crease spreading . The amount of methanol in the fix- flood can be increased to provide faster evaporation , which may be helpful when chromosomes tend to scatter . Dry slides are also ...
Pagina 51
... spreading , and will slow down evaporation . One laboratory in Maryland ( 1 ) re- ports routine use of 6 : 1 fixative in the final fixative because of scattering problems . Barnes and Maltby ( 4 ) recommend use of ethanol instead of ...
... spreading , and will slow down evaporation . One laboratory in Maryland ( 1 ) re- ports routine use of 6 : 1 fixative in the final fixative because of scattering problems . Barnes and Maltby ( 4 ) recommend use of ethanol instead of ...
Pagina 53
... spreading : Al- lowing a drop of cell suspension to touch the water film with the slide flat creates the least spreading , and quickly applying the cells with a squirting technique allows the most spreading . Culturing and Harvesting ...
... spreading : Al- lowing a drop of cell suspension to touch the water film with the slide flat creates the least spreading , and quickly applying the cells with a squirting technique allows the most spreading . Culturing and Harvesting ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL