The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 87
Pagina 99
... tissue culture chamberslides . The number of cultures set up depends on the number of cells that are available . 8. Add 2 mL of MEM Alpha medium with 20 % FBS to the top of each chamberslide . 9. Incubate the cultures at 37 ° C in 5 ...
... tissue culture chamberslides . The number of cultures set up depends on the number of cells that are available . 8. Add 2 mL of MEM Alpha medium with 20 % FBS to the top of each chamberslide . 9. Incubate the cultures at 37 ° C in 5 ...
Pagina 114
... tissue into fine pieces with small scissors in a sterile Petri dish containing a small amount of PBS . 2. Place the cell suspension and clumps in a 125 mL sterile ... tissue culture vessels are not generally employed in the 114 / CHAPTER 4.
... tissue into fine pieces with small scissors in a sterile Petri dish containing a small amount of PBS . 2. Place the cell suspension and clumps in a 125 mL sterile ... tissue culture vessels are not generally employed in the 114 / CHAPTER 4.
Pagina 490
... tissue culture were undergoing at the time . In the late 1960s many tissue culture facilities were switching to less complex , better defined tissue culture media that both enhanced cell growth and allowed a better understanding of the ...
... tissue culture were undergoing at the time . In the late 1960s many tissue culture facilities were switching to less complex , better defined tissue culture media that both enhanced cell growth and allowed a better understanding of the ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL