The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
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Pagina 113
... Trypsin . Because trypsin is inhibited by factors in serum and by the presence of magnesium or calcium ions , all specimens should be washed in a calcium- and magnesium - free BSS without any serum before dis- persal is attempted . The ...
... Trypsin . Because trypsin is inhibited by factors in serum and by the presence of magnesium or calcium ions , all specimens should be washed in a calcium- and magnesium - free BSS without any serum before dis- persal is attempted . The ...
Pagina 134
... trypsin solution at -5 ° to -20 ° C , and thaw it immediately before use . For a working solution , dilute stock trypsin 1:10 with HBSS , Ca2 + - and Mg2 + -free ( room temperature ) . Diluted trypsin may be stored for two to three days ...
... trypsin solution at -5 ° to -20 ° C , and thaw it immediately before use . For a working solution , dilute stock trypsin 1:10 with HBSS , Ca2 + - and Mg2 + -free ( room temperature ) . Diluted trypsin may be stored for two to three days ...
Pagina 170
... Trypsin G - Banding Procedure Contributed by Prenatal Diagnosis Laboratory of New York City PRINCIPLE Chromosome slide preparations are pretreated in a trypsin solution . This sets up the G - banding pattern subsequently revealed with ...
... Trypsin G - Banding Procedure Contributed by Prenatal Diagnosis Laboratory of New York City PRINCIPLE Chromosome slide preparations are pretreated in a trypsin solution . This sets up the G - banding pattern subsequently revealed with ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL