The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
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... ( μg / mL ) 0.012 0.02 Colcemid Time concentration ( μg / mL ) Time Comments 3-4 hr 30 min 0.005 30 min 2 hr 0.02 20 min 3 hr 15 and 30 min 4 hr 30 min 2-4 hr 45 min 0.025 3 hr 0.025 2 hr 0.04 3 hr 0.03 20 min 0.05 30 min 0.05 15-20 min 1 ...
... ( μg / mL ) 0.012 0.02 Colcemid Time concentration ( μg / mL ) Time Comments 3-4 hr 30 min 0.005 30 min 2 hr 0.02 20 min 3 hr 15 and 30 min 4 hr 30 min 2-4 hr 45 min 0.025 3 hr 0.025 2 hr 0.04 3 hr 0.03 20 min 0.05 30 min 0.05 15-20 min 1 ...
Pagina 34
... ( μg / mL ) Time Comments ( μg / mL ) 0.02 10 min 0.012 Time 3-4 hr Comments 15 and 30 min 0.02 40 min 30 min 40 min 0.05 10 min 1 hr 10 min 2-4 hr 10 min 0.025 4-5 hr 10 min 0.05 15 min 0.06 0.1 30 min 1 hr 5 min 10 min 1 hr 2 hr 2-3 hr ...
... ( μg / mL ) Time Comments ( μg / mL ) 0.02 10 min 0.012 Time 3-4 hr Comments 15 and 30 min 0.02 40 min 30 min 40 min 0.05 10 min 1 hr 10 min 2-4 hr 10 min 0.025 4-5 hr 10 min 0.05 15 min 0.06 0.1 30 min 1 hr 5 min 10 min 1 hr 2 hr 2-3 hr ...
Pagina 61
... μg / mL , 39 min TABLE 21. Survey results - combination methods Release time and. Ref . no . Author Tissue type Chemicals used 18 Dewald and Dines pleural AMD a Concentration 5 μg / mL effusions 75 Yunis J.b lymphocytes AMD 5.4 μg / mL ...
... μg / mL , 39 min TABLE 21. Survey results - combination methods Release time and. Ref . no . Author Tissue type Chemicals used 18 Dewald and Dines pleural AMD a Concentration 5 μg / mL effusions 75 Yunis J.b lymphocytes AMD 5.4 μg / mL ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL