The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 246
... vivo methods , or for two cycles , which is easier for in vitro methods . The BrdU method also takes advantage of the fact that the dye 33258 Hoechst fluoresces less efficiently when bound to poly ( dA - dBrdU ) than when bound to poly ...
... vivo methods , or for two cycles , which is easier for in vitro methods . The BrdU method also takes advantage of the fact that the dye 33258 Hoechst fluoresces less efficiently when bound to poly ( dA - dBrdU ) than when bound to poly ...
Pagina 452
... vivo and lengthy transit times to the lab . ( 3 ) Normal cells often contaminate the sample from surrounding tissues or infiltrating inflammatory cells . ( 4 ) Perhaps most im- portantly , at the time of clinical manifestation , most ...
... vivo and lengthy transit times to the lab . ( 3 ) Normal cells often contaminate the sample from surrounding tissues or infiltrating inflammatory cells . ( 4 ) Perhaps most im- portantly , at the time of clinical manifestation , most ...
Pagina 544
... vivo only the epidermis of the volar surfaces produces patterns , in culture the patterns are formed by epidermal cells from EPIDERMAL STRUCTURES ( stratum corneum ) ( stratum germinativum ) . furrow fold glandular fold furrow pore ...
... vivo only the epidermis of the volar surfaces produces patterns , in culture the patterns are formed by epidermal cells from EPIDERMAL STRUCTURES ( stratum corneum ) ( stratum germinativum ) . furrow fold glandular fold furrow pore ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL