In Vitro Cultivation of Micro-organismsElsevier Science & Technology Books, 1992 - 315 pagine A biological examination of the underlying themes to consider how pure cultures of micro-organisms may be isolated from their primary sources to measure their growth and to examine the perameters which influence their performance in culture. |
Dall'interno del libro
Risultati 1-3 di 65
Pagina 68
... measurement of nitrogen content by the Kjeldahl method . This involves chemical digestion of the protein to liberate ammonia which is then measured by titration . The nitrogen content of biomass can be estimated to within 1 % by this ...
... measurement of nitrogen content by the Kjeldahl method . This involves chemical digestion of the protein to liberate ammonia which is then measured by titration . The nitrogen content of biomass can be estimated to within 1 % by this ...
Pagina 69
... Measurement of ATP involves measurement of light produced as a result of an enzyme reaction catalysed by the enzyme firefly luciferase . The amount of light produced is proportional to the amount of ATP taking part in the reaction . The ...
... Measurement of ATP involves measurement of light produced as a result of an enzyme reaction catalysed by the enzyme firefly luciferase . The amount of light produced is proportional to the amount of ATP taking part in the reaction . The ...
Pagina 71
... Measurement of ATP by bioluminescence is more sensitive than the measurement of protein by the Lowry method . 4 ) Wet weight measurements are usually more accurate than dry weight measurements . 5 ) The sensitivity of absorbance ...
... Measurement of ATP by bioluminescence is more sensitive than the measurement of protein by the Lowry method . 4 ) Wet weight measurements are usually more accurate than dry weight measurements . 5 ) The sensitivity of absorbance ...
Sommario
Nutrition and cultivation of microorganisms | 22 |
Summary and objectives | 51 |
The estimation of biomass | 54 |
Copyright | |
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Parole e frasi comuni
aerobic agar amino acids anaerobic antibiotics antimicrobial agent bacteria bacterial cells bacteriophage barophiles batch culture biomass biomass concentration biomass feedback bioreactor buffer carbon source cell cycle cell membrane cell number cell wall chapter chemical chemostat coli components compounds constant containing continuous culture count determined dilution rate disinfectants dissolved oxygen dry weight effect electron energy enzyme Equation Escherichia coli eukaryotic example exponential phase factors fermentation glucose grow growth yield coefficient halophiles heat host cell hydrogen increase incubation infection inhibit isolation limiting nutrient liquid living cells measurement medium mesophiles metabolic method micro-organisms microbial microbiology ml¹ molecular nitrogen nucleic acid number of cells organisms penicillin phage plate production protein psychrophiles replication sample specific growth rate steady-state sterilisation substrate concentration synthesis T4 phage technique temperature thermophiles toxic vessel viral virions virus viruses water activity yeast µmax