Genome Analysis: A Laboratory Manual, Volume 4
A genome revolution is transforming biomedical research in the leading laboratories. Now, a series of manuals from Cold Spring Harbor puts the tools of the revolution in everyone's hands.
Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate expressed sequence to phenotypic variation.
The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness.
A complement to the bible of recombinant DNA, Molecular Cloning, these manuals are essential for every laboratory in which genes are being studied.
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Genetic and Comparative Mapping in Mice
MAPPING A GENE
DNA Markers for Physical Mapping
Representational Difference Analysis
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION
PROCEDURES FOR DIFFERENCE ENRICHMENT
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT
PREPARATION OF DNA PROBES
DENATURATION HYBRIDIZATION AND WASHING
STAINING CHROMOSOMES AND CHROMOSOME BANDS
Somatic Cell Genetics and Radiation Hybrid Mapping
Fluorescence In Situ Hybridization
FISH PROBES AND COUNTERSTAINS
MAPPING APPLICATIONS FOR FISH