Genome Analysis: A Laboratory Manual, Volume 4

Copertina anteriore
Bruce Birren, Eric D. Green, Sue Klapholz
CSHL Press, 1997 - 494 pagine
A genome revolution is transforming biomedical research in the leading laboratories. Now, a series of manuals from Cold Spring Harbor puts the tools of the revolution in everyone's hands.

Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate expressed sequence to phenotypic variation.

The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness.

A complement to the bible of recombinant DNA, Molecular Cloning, these manuals are essential for every laboratory in which genes are being studied.

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Sommario

Meiotic Mapping in Humans
1
Genetic and Comparative Mapping in Mice
71
MAPPING A GENE
88
DNA Markers for Physical Mapping
187
Representational Difference Analysis
217
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION
223
PROCEDURES FOR DIFFERENCE ENRICHMENT
242
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT
252
FISH PROCEDURES
333
PREPARATION OF DNA PROBES
351
DENATURATION HYBRIDIZATION AND WASHING
372
IMMUNOCYTOCHEMICAL DETECTION
384
STAINING CHROMOSOMES AND CHROMOSOME BANDS
391
MICROSCOPY
405
Common Reagents
415
Basic Procedures
439

Somatic Cell Genetics and Radiation Hybrid Mapping
259
Fluorescence In Situ Hybridization
303
FISH PROBES AND COUNTERSTAINS
313
MAPPING APPLICATIONS FOR FISH
327
Safety Cautions
465
Useful Facts
473
Suppliers
481
Copyright

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