Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-3 di 3
Pagina 12
... amount of information available from a single microsatel- lite marker . ( 6 ) Statistical methods are then used to evaluate the probability that the phenotype of interest maps with certainty to the marker loci that have been genotyped ...
... amount of information available from a single microsatel- lite marker . ( 6 ) Statistical methods are then used to evaluate the probability that the phenotype of interest maps with certainty to the marker loci that have been genotyped ...
Pagina 14
... amount of data nec- essary to detect linkage to multiple loci and the required resolution . The current major challenge , and indeed much of the contemporary statistical developments in linkage analysis , is the elucidation of the ...
... amount of data nec- essary to detect linkage to multiple loci and the required resolution . The current major challenge , and indeed much of the contemporary statistical developments in linkage analysis , is the elucidation of the ...
Pagina 15
... amount of data necessary to map complex traits depends on all of these initially unknown factors . Much of current research is geared to the meth- ods of mapping that can be successful in the face of this ignorance ; all of these ...
... amount of data necessary to map complex traits depends on all of these initially unknown factors . Much of current research is geared to the meth- ods of mapping that can be successful in the face of this ignorance ; all of these ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
Altre edizioni - Visualizza tutto
Parole e frasi comuni
20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
Brani popolari
Pagina 214 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Pagina 66 - J. (1991 ). Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer's disease.
Pagina 132 - ES 1992. A genetic map of the mouse suitable for typing intraspecific crosses.
Pagina 67 - Levy-Lahad, E., Wasco, W., Poorkaj, P., Romano, DM, Oshima, J., Pettingell, WH, Yu...
Pagina 182 - Green, P. (1998) Base-calling of automated sequencer traces using Phred. I. Accuracy assessment.
Pagina 257 - E. Cesarman, MS Pessin, F. Lee, J. Culpepper, DM Knowles, and PS Moore, "Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma," Science, 266, 1865-1869, 1994.