Genome Analysis: A Laboratory Manual, Volume 4

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Eric D. Green
CSHL Press, 1997 - 494 pagine
The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness.

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Sommario

Meiotic Mapping in Humans
1
HUMAN PEDIGREES
16
INHERITANCE PATTERN OF THE PHENOTYPE
24
GENETIC MARKERS
33
Genetic and Comparative Mapping in Mice
71
MAPPING A GENE
88
BACKCROSS ANALYSIS
113
INTERCROSS ANALYSIS
120
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT
252
Somatic Cell Genetics and Radiation Hybrid Mapping
259
INTERSPECIFIC CELL HYBRIDS
268
Fluorescence In Situ Hybridization
303
FISH PROBES AND COUNTERSTAINS
313
MAPPING APPLICATIONS FOR FISH
327
FISH PROCEDURES
333
PREPARATION OF DNA PROBES
351

Identification and Analysis of DNA Polymorphisms
135
IDENTIFICATION OF SNPs
144
ANALYSIS OF SNPs
153
IDENTIFICATION OF STRPs
167
DNA Markers for Physical Mapping
187
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES
193
Purification of YAC DNA by PFGE AND Electroelution
203
Representational Difference Analysis
217
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION
223
PROCEDURES FOR DIFFERENCE ENRICHMENT
242
DENATURATION HYBRIDIZATION AND WASHING
372
IMMUNOCYTOCHEMICAL DETECTION
384
STAINING CHROMOSOMES AND CHROMOSOME BANDS
391
MICROSCOPY
405
Common Reagents
415
Basic Procedures
439
Safety Cautions
465
Useful Facts
473
Suppliers
481
Copyright

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