Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 6
Pagina 12
... Table 1A , B . Genome Mapping and Genome Scanning The seminal paper by Botstein et al . ( 1980 ) introduced the concept of construct- ing a human genome - wide linkage map using polymorphic DNA markers that Figure 4 Outline of a meiotic ...
... Table 1A , B . Genome Mapping and Genome Scanning The seminal paper by Botstein et al . ( 1980 ) introduced the concept of construct- ing a human genome - wide linkage map using polymorphic DNA markers that Figure 4 Outline of a meiotic ...
Pagina 16
... Table 2 lists the characteristics of these CEPH reference pedigrees . DNA samples from these families have been sent to more than 100 collaborating scientists to develop the human genetic linkage map . The large number of samples in the ...
... Table 2 lists the characteristics of these CEPH reference pedigrees . DNA samples from these families have been sent to more than 100 collaborating scientists to develop the human genetic linkage map . The large number of samples in the ...
Pagina 23
... Table 1 for all programs mentioned in the text ) . These systems maintain and manage genetic data , are useful for preparation of reports , and can automatically generate the computer files necessary for a vari- ety of linkage analyses ...
... Table 1 for all programs mentioned in the text ) . These systems maintain and manage genetic data , are useful for preparation of reports , and can automatically generate the computer files necessary for a vari- ety of linkage analyses ...
Pagina 29
... Table 1 ) POINTER ( Lalouel and Morton 1981 ; Lalouel et al . 1983 ) and PAP ( Hasstedt and Cartwright 1981 ) are available at no charge for performing these analyses ; PAP also allows tests of oligogenic ( traits produced by just a few ...
... Table 1 ) POINTER ( Lalouel and Morton 1981 ; Lalouel et al . 1983 ) and PAP ( Hasstedt and Cartwright 1981 ) are available at no charge for performing these analyses ; PAP also allows tests of oligogenic ( traits produced by just a few ...
Pagina 42
... Table 1 ) . Each of these programs performs additional analyses and has their individual advantages and disadvantages for pair - wise linkage studies . For genome mapping involving many markers , CRI - MAP and LINKAGE are the most ...
... Table 1 ) . Each of these programs performs additional analyses and has their individual advantages and disadvantages for pair - wise linkage studies . For genome mapping involving many markers , CRI - MAP and LINKAGE are the most ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
Altre edizioni - Visualizza tutto
Parole e frasi comuni
20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
Brani popolari
Pagina 214 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Pagina 66 - J. (1991 ). Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer's disease.
Pagina 132 - ES 1992. A genetic map of the mouse suitable for typing intraspecific crosses.
Pagina 67 - Levy-Lahad, E., Wasco, W., Poorkaj, P., Romano, DM, Oshima, J., Pettingell, WH, Yu...
Pagina 182 - Green, P. (1998) Base-calling of automated sequencer traces using Phred. I. Accuracy assessment.
Pagina 257 - E. Cesarman, MS Pessin, F. Lee, J. Culpepper, DM Knowles, and PS Moore, "Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma," Science, 266, 1865-1869, 1994.