Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 12
Pagina v
... Construction in Humans , 6 Advances in Human Linkage Data Analysis , 8 Genome Mapping and Genome Scanning , 12 HUMAN PEDIGREES , 16 Reference Pedigrees for Genome Mapping , 16 Sampling Disease Families , 18 Family Studies , 22 ...
... Construction in Humans , 6 Advances in Human Linkage Data Analysis , 8 Genome Mapping and Genome Scanning , 12 HUMAN PEDIGREES , 16 Reference Pedigrees for Genome Mapping , 16 Sampling Disease Families , 18 Family Studies , 22 ...
Pagina 1
... construction of comprehensive human genet- ic maps . A large number of disease genes with Mendelian phenotypes have been localized on the human linkage map as a prelude to positional cloning . This map is proving to be increas- ingly ...
... construction of comprehensive human genet- ic maps . A large number of disease genes with Mendelian phenotypes have been localized on the human linkage map as a prelude to positional cloning . This map is proving to be increas- ingly ...
Pagina 2
... construction of linkage maps of chromosomes . These principles have held true for the mapping of human chromosomes . A Brief History of Human Genetic Mapping The first gene to be mapped in the human was that for color blindness ...
... construction of linkage maps of chromosomes . These principles have held true for the mapping of human chromosomes . A Brief History of Human Genetic Mapping The first gene to be mapped in the human was that for color blindness ...
Pagina 6
... Construction in Humans Linkage or meiotic mapping , in principle , is straightforward . The essence of mei- otic mapping is the estimation of the frequency of recombination between two loci ; this frequency increases as the distance ...
... Construction in Humans Linkage or meiotic mapping , in principle , is straightforward . The essence of mei- otic mapping is the estimation of the frequency of recombination between two loci ; this frequency increases as the distance ...
Pagina 10
... construction haplotype reconstruction in pedigrees based on the Elston - Stewart algorithm analyze heterogeneity with respect to a single - marker locus sib - pair tests ( APM and Haseman - Elston ) , TDT and association tests haplotype ...
... construction haplotype reconstruction in pedigrees based on the Elston - Stewart algorithm analyze heterogeneity with respect to a single - marker locus sib - pair tests ( APM and Haseman - Elston ) , TDT and association tests haplotype ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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