Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 6
Pagina 7
... crosses in experimental organisms where a large number of meioses could be assayed from a single mating , human geneticists rec- ognized the need to obtain a large number of human families ( each with a few meioses ) for linkage ...
... crosses in experimental organisms where a large number of meioses could be assayed from a single mating , human geneticists rec- ognized the need to obtain a large number of human families ( each with a few meioses ) for linkage ...
Pagina 8
... crosses , since a cross with multiple infor- mative markers was difficult to generate . Thus , there were no concerted efforts Family 1 : 2 2 3 6 # # 6 8 Chapter 1☐ Meiotic Mapping in Humans Advances in Human Linkage Data Analysis,
... crosses , since a cross with multiple infor- mative markers was difficult to generate . Thus , there were no concerted efforts Family 1 : 2 2 3 6 # # 6 8 Chapter 1☐ Meiotic Mapping in Humans Advances in Human Linkage Data Analysis,
Pagina 11
... crosses automated construction of linkage maps ( uses CRI - MAP ) PEDSYS $ management of genetic data PROGENY $ pedigree drawing linked to a Sybase database ( D ) Segregation analysis programs COMDS combined segregation and linkage ...
... crosses automated construction of linkage maps ( uses CRI - MAP ) PEDSYS $ management of genetic data PROGENY $ pedigree drawing linked to a Sybase database ( D ) Segregation analysis programs COMDS combined segregation and linkage ...
Pagina 38
... cross , for the case of mapping a rare dominant trait . The theoretical formula for the PIC can be reduced to : k k k PIC = 1 - Σ - Σ ΣΣΣ i = 1 i = 1 i = 1 4 which for a highly polymorphic locus will generate a value only slightly ...
... cross , for the case of mapping a rare dominant trait . The theoretical formula for the PIC can be reduced to : k k k PIC = 1 - Σ - Σ ΣΣΣ i = 1 i = 1 i = 1 4 which for a highly polymorphic locus will generate a value only slightly ...
Pagina 42
... crosses , such as mice . For these situations , it has the advantage of implementing an algorithm for de novo linkage group construction : As long as some " anchor " loci have previous- ly been assigned to specific chromosomes ...
... crosses , such as mice . For these situations , it has the advantage of implementing an algorithm for de novo linkage group construction : As long as some " anchor " loci have previous- ly been assigned to specific chromosomes ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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