Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 17
Pagina 2
... detection of linkage have been available since 1955 ( Morton 1955 ) , the assignment of linkage groups to chromosomes , a step that would allow the genetic mapping of entire chromo- 1. Autosomal dominant 11. Autosomal recessive A III ...
... detection of linkage have been available since 1955 ( Morton 1955 ) , the assignment of linkage groups to chromosomes , a step that would allow the genetic mapping of entire chromo- 1. Autosomal dominant 11. Autosomal recessive A III ...
Pagina 4
... detection of nonallelic heterogeneity ( where more than one mutant gene can cause the same phenotype ) as first demonstrated for hereditary elliptocytosis by Morton ( 1956 ) . This is important not only for the " nosology " ( or ...
... detection of nonallelic heterogeneity ( where more than one mutant gene can cause the same phenotype ) as first demonstrated for hereditary elliptocytosis by Morton ( 1956 ) . This is important not only for the " nosology " ( or ...
Pagina 7
... detection and then study only those that were informative and in which linkage phase could be determined . Thus , linkage evidence in humans had to be collected from a number of different natural crosses with dif- ferent linkage phases ...
... detection and then study only those that were informative and in which linkage phase could be determined . Thus , linkage evidence in humans had to be collected from a number of different natural crosses with dif- ferent linkage phases ...
Pagina 8
... detection of linkage between two markers is essentially a test of intrafamily association between the markers . This association is tested by examining the probability that the alleles at two loci transmitted to a parent by a specific ...
... detection of linkage between two markers is essentially a test of intrafamily association between the markers . This association is tested by examining the probability that the alleles at two loci transmitted to a parent by a specific ...
Pagina 10
... detecting linkage given a set of Web / ftp / email Location http://www.mayo.edu/statgen ftp://ftp-genome.wi.mit.edu ... detection in reference pedigrees linkage disequilibrium test for multiple loci pair - wise linkage disequilibrium ...
... detecting linkage given a set of Web / ftp / email Location http://www.mayo.edu/statgen ftp://ftp-genome.wi.mit.edu ... detection in reference pedigrees linkage disequilibrium test for multiple loci pair - wise linkage disequilibrium ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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