Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 28
Pagina v
... Disease Families , 18 Family Studies , 22 INHERITANCE PATTERN OF THE PHENOTYPE , 24 Mendelian Traits , 24 Phenotypic Variation in Mendelian Traits , 25 Segregation Analysis , 27 Enrichment for Genetic Causation , 30 GENETIC MARKERS , 33 ...
... Disease Families , 18 Family Studies , 22 INHERITANCE PATTERN OF THE PHENOTYPE , 24 Mendelian Traits , 24 Phenotypic Variation in Mendelian Traits , 25 Segregation Analysis , 27 Enrichment for Genetic Causation , 30 GENETIC MARKERS , 33 ...
Pagina 1
... disease genes with Mendelian phenotypes have been localized on the human linkage map as a prelude to positional cloning . This map is proving to be increas- ingly useful in establishing and elucidating the genetic basis of a number of ...
... disease genes with Mendelian phenotypes have been localized on the human linkage map as a prelude to positional cloning . This map is proving to be increas- ingly useful in establishing and elucidating the genetic basis of a number of ...
Pagina 4
... disease , in genetic counseling localize hereditary disorders to specific regions of the genome clone genes by the positional cloning and positional candidate approach strategies ⚫ study gene conservation across species and investigate ...
... disease , in genetic counseling localize hereditary disorders to specific regions of the genome clone genes by the positional cloning and positional candidate approach strategies ⚫ study gene conservation across species and investigate ...
Pagina 5
... disease gene , has led to a new and extremely successful paradigm called positional cloning ( Ballabio et al . 1998 , Vol . 2 ) . Beginning with the cloning of chronic granuloma- tous disease in 1986 ( Royer - Pokora et al . 1986 ) ...
... disease gene , has led to a new and extremely successful paradigm called positional cloning ( Ballabio et al . 1998 , Vol . 2 ) . Beginning with the cloning of chronic granuloma- tous disease in 1986 ( Royer - Pokora et al . 1986 ) ...
Pagina 11
... following the name of the software indicates that a fee is charged for acquisition of this software . 1. Identify families segregating the disease of interest . Mendelian Background , History , and Current Status of Human Genetic Mapping ...
... following the name of the software indicates that a fee is charged for acquisition of this software . 1. Identify families segregating the disease of interest . Mendelian Background , History , and Current Status of Human Genetic Mapping ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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