Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 8
Pagina 2
... efficient statistical methods for the detection of linkage have been available since 1955 ( Morton 1955 ) , the assignment of linkage groups to chromosomes , a step that would allow the genetic mapping of entire chromo- 1. Autosomal ...
... efficient statistical methods for the detection of linkage have been available since 1955 ( Morton 1955 ) , the assignment of linkage groups to chromosomes , a step that would allow the genetic mapping of entire chromo- 1. Autosomal ...
Pagina 8
... efficient mapping methods for multiple markers . Although the first estimate of the frequency of crossing over between two human genes occurred in 1947 ( Haldane and Smith 1947 ) , it took another 40 years to resolve the issues that ...
... efficient mapping methods for multiple markers . Although the first estimate of the frequency of crossing over between two human genes occurred in 1947 ( Haldane and Smith 1947 ) , it took another 40 years to resolve the issues that ...
Pagina 12
... Efficient lod score computations in arbitrary pedigrees were introduced by J. Ott in 1974 and were based on the ... efficiency of map construction . For a list of currently available computer programs for linkage analysis and map ...
... Efficient lod score computations in arbitrary pedigrees were introduced by J. Ott in 1974 and were based on the ... efficiency of map construction . For a list of currently available computer programs for linkage analysis and map ...
Pagina 19
... efficient when a collection of large pedigrees with multiple generations of affected individuals are available . Although a single large kindred may be sufficient for linkage mapping of a dis- ease gene , a number of pedigrees are ...
... efficient when a collection of large pedigrees with multiple generations of affected individuals are available . Although a single large kindred may be sufficient for linkage mapping of a dis- ease gene , a number of pedigrees are ...
Pagina 22
... efficient statistical methods for linkage detection in affected sibling pairs have now been devised . Family Studies ... efficiency . Krush and Evans ( 1984 ) have provided guidelines for these studies . Phenotype data should be reliably ...
... efficient statistical methods for linkage detection in affected sibling pairs have now been devised . Family Studies ... efficiency . Krush and Evans ( 1984 ) have provided guidelines for these studies . Phenotype data should be reliably ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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