Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 18
Pagina 1
... frequency with which this recom- bination occurs between two distinct markers depends primarily not only on the length of the chromosomal segment between the markers , but also on the specific nucleotide sequence and its location in the ...
... frequency with which this recom- bination occurs between two distinct markers depends primarily not only on the length of the chromosomal segment between the markers , but also on the specific nucleotide sequence and its location in the ...
Pagina 2
... frequency ( for an introduction to basic terminology in genetics , see Reeves and D'Eustachio , this volume ) . To assess recombination in the gametes of an individual , the homolo- gous chromosomes of that individual must be ...
... frequency ( for an introduction to basic terminology in genetics , see Reeves and D'Eustachio , this volume ) . To assess recombination in the gametes of an individual , the homolo- gous chromosomes of that individual must be ...
Pagina 6
... frequency of recombination between two loci ; this frequency increases as the distance between the loci increases ( Figure 2 ) . To obtain a high - density linkage map in which the intermarker distances are small , a large number of ...
... frequency of recombination between two loci ; this frequency increases as the distance between the loci increases ( Figure 2 ) . To obtain a high - density linkage map in which the intermarker distances are small , a large number of ...
Pagina 7
... frequencies . In this illustration , the number of recombination events per chromosome varies from zero to two observed recombinations . The frequency and placement of recombi- nation events depend on many factors , including the ...
... frequencies . In this illustration , the number of recombination events per chromosome varies from zero to two observed recombinations . The frequency and placement of recombi- nation events depend on many factors , including the ...
Pagina 8
... frequency of crossing over between two human genes occurred in 1947 ( Haldane and Smith 1947 ) , it took another 40 years to resolve the issues that challenged human genetic mapping . Much of this progress has occurred in the last ...
... frequency of crossing over between two human genes occurred in 1947 ( Haldane and Smith 1947 ) , it took another 40 years to resolve the issues that challenged human genetic mapping . Much of this progress has occurred in the last ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
Altre edizioni - Visualizza tutto
Parole e frasi comuni
20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
Brani popolari
Pagina 214 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Pagina 66 - J. (1991 ). Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer's disease.
Pagina 132 - ES 1992. A genetic map of the mouse suitable for typing intraspecific crosses.
Pagina 67 - Levy-Lahad, E., Wasco, W., Poorkaj, P., Romano, DM, Oshima, J., Pettingell, WH, Yu...
Pagina 182 - Green, P. (1998) Base-calling of automated sequencer traces using Phred. I. Accuracy assessment.
Pagina 257 - E. Cesarman, MS Pessin, F. Lee, J. Culpepper, DM Knowles, and PS Moore, "Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma," Science, 266, 1865-1869, 1994.