Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 26
Pagina v
... Humans , 1 A. Chakravarti , A. Lynn BACKGROUND , HISTORY , AND CURRENT STATUS OF HUMAN GENETIC MAPPING , 2 A Brief History of Human Genetic Mapping , 2 Applications of a Linkage Map , 4 The Challenges of Map Construction in Humans , 6 ...
... Humans , 1 A. Chakravarti , A. Lynn BACKGROUND , HISTORY , AND CURRENT STATUS OF HUMAN GENETIC MAPPING , 2 A Brief History of Human Genetic Mapping , 2 Applications of a Linkage Map , 4 The Challenges of Map Construction in Humans , 6 ...
Pagina 1
... human beings during female and male meiosis , homologous chromosomal segments are fre- quently exchanged to produce recombined segments . The frequency with which this recom ... HUMAN GENETIC Meiotic Mapping in Humans, A Chakravarti, A Lynn.
... human beings during female and male meiosis , homologous chromosomal segments are fre- quently exchanged to produce recombined segments . The frequency with which this recom ... HUMAN GENETIC Meiotic Mapping in Humans, A Chakravarti, A Lynn.
Pagina 2
... HUMAN GENETIC MAPPING Mapping entails assigning a human gene or locus to a specific region of the human genome , generally a chromosome or a chromosomal band . In contrast , genetic mapping ( also known as linkage or meiotic mapping ) ...
... HUMAN GENETIC MAPPING Mapping entails assigning a human gene or locus to a specific region of the human genome , generally a chromosome or a chromosomal band . In contrast , genetic mapping ( also known as linkage or meiotic mapping ) ...
Pagina 3
... human genome , remained an opportunistic activity because of a lack of detectable physical landmarks on individual chromosomes . However , this problem was drastically altered when Weiss and Green ( 1967 ) demonstrated the feasibility ...
... human genome , remained an opportunistic activity because of a lack of detectable physical landmarks on individual chromosomes . However , this problem was drastically altered when Weiss and Green ( 1967 ) demonstrated the feasibility ...
Pagina 5
... human genome , as a prelude to gene identification . For a large number of human genetic diseases , the mode of inheritance can be inferred from inspection of families in which the disorder is clearly segregating . Knowledge of the mode ...
... human genome , as a prelude to gene identification . For a large number of human genetic diseases , the mode of inheritance can be inferred from inspection of families in which the disorder is clearly segregating . Knowledge of the mode ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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