Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 25
Pagina 2
... individual , the homolo- gous chromosomes of that individual must be differentially recognized or marked ; thus , linkage mapping can only use those loci that demonstrate poly- morphic variation . Thus , any polymorphic locus , defined ...
... individual , the homolo- gous chromosomes of that individual must be differentially recognized or marked ; thus , linkage mapping can only use those loci that demonstrate poly- morphic variation . Thus , any polymorphic locus , defined ...
Pagina 3
... individual chromosomes . However , this problem was drastically altered when Weiss and Green ( 1967 ) demonstrated the feasibility of gene assignment to specific chromosomes , or subregions of chromo- somes , by using interspecies ...
... individual chromosomes . However , this problem was drastically altered when Weiss and Green ( 1967 ) demonstrated the feasibility of gene assignment to specific chromosomes , or subregions of chromo- somes , by using interspecies ...
Pagina 4
... individual can be greatly improved ( Chakravarti and Nei 1982 ) ( see p . 30 ) . This feature was recognized shortly after J.B.S. Haldane established the close linkage between color blindness and hemophilia in 1937 , when Hoogvliet ...
... individual can be greatly improved ( Chakravarti and Nei 1982 ) ( see p . 30 ) . This feature was recognized shortly after J.B.S. Haldane established the close linkage between color blindness and hemophilia in 1937 , when Hoogvliet ...
Pagina 5
... individuals , only a probability of affection can be calculated and presymptomatic or antenatal diagnosis of the disorder is not possible . Knowledge of the specific gene involved is extremely helpful not only for diagnosis , but also ...
... individuals , only a probability of affection can be calculated and presymptomatic or antenatal diagnosis of the disorder is not possible . Knowledge of the specific gene involved is extremely helpful not only for diagnosis , but also ...
Pagina 12
... individuals . However , if the disease under study does not have a clear mode of inheritance or if age of onset is late ( i.e. , in situations where it is likely that parents and other ances- tors will be deceased ) , a sib pair or case ...
... individuals . However , if the disease under study does not have a clear mode of inheritance or if age of onset is late ( i.e. , in situations where it is likely that parents and other ances- tors will be deceased ) , a sib pair or case ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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