Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 36
Pagina v
... Linkage Map , 4 The Challenges of Map Construction in Humans , 6 Advances in Human Linkage Data Analysis , 8 Genome Mapping and Genome Scanning , 12 HUMAN PEDIGREES , 16 Reference Pedigrees for Genome Mapping , 16 Sampling Disease ...
... Linkage Map , 4 The Challenges of Map Construction in Humans , 6 Advances in Human Linkage Data Analysis , 8 Genome Mapping and Genome Scanning , 12 HUMAN PEDIGREES , 16 Reference Pedigrees for Genome Mapping , 16 Sampling Disease ...
Pagina 2
... linkage or meiotic mapping ) is the determina- tion of the linear arrangement of genes or loci on a chromosome separated by distances that are measured in units of meiotic recombination frequency ( for an introduction to basic ...
... linkage or meiotic mapping ) is the determina- tion of the linear arrangement of genes or loci on a chromosome separated by distances that are measured in units of meiotic recombination frequency ( for an introduction to basic ...
Pagina 3
... Linkage mapping together with somatic cell hybrid mapping subsequently led to great activity in gene mapping in the 1970s . By 1976 , at least one gene had been mapped to each of the 22 autosomes and the X and Y chromosomes , and some ...
... Linkage mapping together with somatic cell hybrid mapping subsequently led to great activity in gene mapping in the 1970s . By 1976 , at least one gene had been mapped to each of the 22 autosomes and the X and Y chromosomes , and some ...
Pagina 4
... Linkage Map A linkage map can be used to : · establish the genetic basis of traits , i.e. , whether Mendelian or non- Mendelian or under the control of multigenic factors predict risk for disease , in genetic counseling localize ...
... Linkage Map A linkage map can be used to : · establish the genetic basis of traits , i.e. , whether Mendelian or non- Mendelian or under the control of multigenic factors predict risk for disease , in genetic counseling localize ...
Pagina 5
... linkage information is increasingly being applied to family studies . The primary , most frequent application of a linkage map is in the localization of a hereditary disorder to a small segment of the human genome , as a prelude to gene ...
... linkage information is increasingly being applied to family studies . The primary , most frequent application of a linkage map is in the localization of a hereditary disorder to a small segment of the human genome , as a prelude to gene ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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