Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 25
Pagina 1
... markers depends primarily not only on the length of the chromosomal segment between the markers , but also on the specific nucleotide sequence and its location in the genome , and whether the process occurs in the female or in the male ...
... markers depends primarily not only on the length of the chromosomal segment between the markers , but also on the specific nucleotide sequence and its location in the genome , and whether the process occurs in the female or in the male ...
Pagina 4
... markers with an average spacing of 1 cM , i.e. , 1 Mb , or less . The identification of genetic markers closely linked to a disease is of direct use in genetic counseling because the accuracy of risk prediction in relatives of an ...
... markers with an average spacing of 1 cM , i.e. , 1 Mb , or less . The identification of genetic markers closely linked to a disease is of direct use in genetic counseling because the accuracy of risk prediction in relatives of an ...
Pagina 6
... markers being mapped be heterozygous ( i.e. , the two alleles at each locus be distinguishable from each other ) in the diploid parent whose meioses are being examined ( such an indi- vidual is termed " informative " ; see pp . 37–38 ) ...
... markers being mapped be heterozygous ( i.e. , the two alleles at each locus be distinguishable from each other ) in the diploid parent whose meioses are being examined ( such an indi- vidual is termed " informative " ; see pp . 37–38 ) ...
Pagina 8
... markers is essentially a test of intrafamily association between the markers . This association is tested by examining the probability that the alleles at two loci transmitted to a parent by a specific grand- parent , e.g. , the ...
... markers is essentially a test of intrafamily association between the markers . This association is tested by examining the probability that the alleles at two loci transmitted to a parent by a specific grand- parent , e.g. , the ...
Pagina 9
... markers . ( Family 1 ) Of 12 meioses , 2 are recombinant . This is much less than 6 of the 12 predicted meioses if the loci were segregating randomly ( i.e. , unlinked ) . ( Family 2 ) Of 12 meioses , 6 are recombinant , thus giving the ...
... markers . ( Family 1 ) Of 12 meioses , 2 are recombinant . This is much less than 6 of the 12 predicted meioses if the loci were segregating randomly ( i.e. , unlinked ) . ( Family 2 ) Of 12 meioses , 6 are recombinant , thus giving the ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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