Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 15
Pagina 4
... mutant gene can cause the same phenotype ) as first demonstrated for hereditary elliptocytosis by Morton ( 1956 ) . This is important not only for the " nosology " ( or clinical charac- terization ) of the disorder and for genetic ...
... mutant gene can cause the same phenotype ) as first demonstrated for hereditary elliptocytosis by Morton ( 1956 ) . This is important not only for the " nosology " ( or clinical charac- terization ) of the disorder and for genetic ...
Pagina 5
... of chromosomal evolution , as well as have a direct benefit in human genetic analysis by identifying candidate homologous mutant phenotypes in the mouse Background , History , and Current Status of Human Genetic Mapping 5.
... of chromosomal evolution , as well as have a direct benefit in human genetic analysis by identifying candidate homologous mutant phenotypes in the mouse Background , History , and Current Status of Human Genetic Mapping 5.
Pagina 6
A Laboratory Manual Eric D. Green. analysis by identifying candidate homologous mutant phenotypes in the mouse . The application of linkage maps to the solution is still in its infancy but is likely to gain favor as meiotic maps for ...
A Laboratory Manual Eric D. Green. analysis by identifying candidate homologous mutant phenotypes in the mouse . The application of linkage maps to the solution is still in its infancy but is likely to gain favor as meiotic maps for ...
Pagina 14
... occurrence of phenocopies ( individuals who are affected due to a different cause , such as an environmental exposure ) , reduced penetrance ( some individuals who carry the mutation but do 14 Chapter 1 Meiotic Mapping in Humans.
... occurrence of phenocopies ( individuals who are affected due to a different cause , such as an environmental exposure ) , reduced penetrance ( some individuals who carry the mutation but do 14 Chapter 1 Meiotic Mapping in Humans.
Pagina 15
A Laboratory Manual Eric D. Green. penetrance ( some individuals who carry the mutation but do not manifest the disease phenotype ) , and epistatic interaction between genes ( the effect of two or more genes acting together or against ...
A Laboratory Manual Eric D. Green. penetrance ( some individuals who carry the mutation but do not manifest the disease phenotype ) , and epistatic interaction between genes ( the effect of two or more genes acting together or against ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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