Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 21
Pagina v
... 25 Segregation Analysis , 27 Enrichment for Genetic Causation , 30 GENETIC MARKERS , 33 Polymorphic Markers : Tools for Detecting DNA Variation , 33 Calculating Heterozygosity , 36 Informative Meioses , 37 Haplotypes and.
... 25 Segregation Analysis , 27 Enrichment for Genetic Causation , 30 GENETIC MARKERS , 33 Polymorphic Markers : Tools for Detecting DNA Variation , 33 Calculating Heterozygosity , 36 Informative Meioses , 37 Haplotypes and.
Pagina 1
... polymorphic gene , or a Mendelian phenotype is localized within a genome on the basis of its pattern of segregation with respect to another marker locus or a set of loci . In human beings during female and male meiosis , homologous ...
... polymorphic gene , or a Mendelian phenotype is localized within a genome on the basis of its pattern of segregation with respect to another marker locus or a set of loci . In human beings during female and male meiosis , homologous ...
Pagina 2
... polymorphic locus , defined as a locus segregating multiple alleles in the population ( with the frequency of the least common allele exceeding 1 % ) , is also called a genetic marker ( see p . 33 ) . Although linkage map- ping can ...
... polymorphic locus , defined as a locus segregating multiple alleles in the population ( with the frequency of the least common allele exceeding 1 % ) , is also called a genetic marker ( see p . 33 ) . Although linkage map- ping can ...
Pagina 3
... in linkage mapping did not occur until the late 1980s and awaited the advent of molecular genetics and the large - scale development of polymorphic DNA markers ( Background , History , and Current Status of Human Genetic Mapping 3.
... in linkage mapping did not occur until the late 1980s and awaited the advent of molecular genetics and the large - scale development of polymorphic DNA markers ( Background , History , and Current Status of Human Genetic Mapping 3.
Pagina 4
A Laboratory Manual Eric D. Green. the large - scale development of polymorphic DNA markers ( see pp . 31-36 ; Botstein et al . 1980 ; Wyman and White 1980 ; Litt and Luty 1989 ; Weber 1990 ) . Applications of a Linkage Map A linkage map ...
A Laboratory Manual Eric D. Green. the large - scale development of polymorphic DNA markers ( see pp . 31-36 ; Botstein et al . 1980 ; Wyman and White 1980 ; Litt and Luty 1989 ; Weber 1990 ) . Applications of a Linkage Map A linkage map ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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