Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 9
Pagina 8
... sample statistical test . Second , Morton introduced the edict that an incorrect linkage map is worse than no linkage map at all and pointed out the low prior chance ( 5 % ) of linkage between any two loci : These issues determined ...
... sample statistical test . Second , Morton introduced the edict that an incorrect linkage map is worse than no linkage map at all and pointed out the low prior chance ( 5 % ) of linkage between any two loci : These issues determined ...
Pagina 10
... sample of affected sibling pairs error detection in reference pedigrees linkage disequilibrium test for multiple loci pair - wise linkage disequilibrium tests nonparametric analysis using extended relative pairs multi - allele TDT tests ...
... sample of affected sibling pairs error detection in reference pedigrees linkage disequilibrium test for multiple loci pair - wise linkage disequilibrium tests nonparametric analysis using extended relative pairs multi - allele TDT tests ...
Pagina 16
... samples from all four grandparents , both parents , and a large sibship size , were introduced to remedy this defect ... samples from these families have been sent to more than 100 collaborating scientists to develop the human genetic ...
... samples from all four grandparents , both parents , and a large sibship size , were introduced to remedy this defect ... samples from these families have been sent to more than 100 collaborating scientists to develop the human genetic ...
Pagina 18
... Sampling Disease Families ASCERTAINING PROBANDS AND THEIR FAMILIES All disease families are ascertained to be ... sampling structure , such as ascertainment of the nuclear family containing the proband , or a variable sampling structure ...
... Sampling Disease Families ASCERTAINING PROBANDS AND THEIR FAMILIES All disease families are ascertained to be ... sampling structure , such as ascertainment of the nuclear family containing the proband , or a variable sampling structure ...
Pagina 19
... sampling strategies for identifying Mendelian disease families . First , the usual approach is to sample a large number of independent nuclear families ( two parents and all of their affected and unaffected offspring ) or two ...
... sampling strategies for identifying Mendelian disease families . First , the usual approach is to sample a large number of independent nuclear families ( two parents and all of their affected and unaffected offspring ) or two ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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