Genome Analysis: A Laboratory Manual, Volume 4Eric D. Green CSHL Press, 1997 - 494 pagine The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. |
Dall'interno del libro
Risultati 1-5 di 9
Pagina 8
... score , " which is the base - 10 logarithm of the ratio of the likelihood ( or probability ) of the joint segregation of two loci under the assump- tions of linkage and no linkage , respectively ( see pp . 41-42 ) . In a remarkable ...
... score , " which is the base - 10 logarithm of the ratio of the likelihood ( or probability ) of the joint segregation of two loci under the assump- tions of linkage and no linkage , respectively ( see pp . 41-42 ) . In a remarkable ...
Pagina 10
... scores ; two - locus or multi - locus likelihood calculations in pedigrees , two - locus only multipoint lod score calculations for families with inbreeding loops calculates the probability distribution for IBD status for points along a ...
... scores ; two - locus or multi - locus likelihood calculations in pedigrees , two - locus only multipoint lod score calculations for families with inbreeding loops calculates the probability distribution for IBD status for points along a ...
Pagina 12
... score computations in arbitrary pedigrees were introduced by J. Ott in 1974 and were based on the powerful Elston - Stewart algorithm ( 1971 ) , which allowed likeli- hood calculations in pedigrees . The popularity of linkage analysis ...
... score computations in arbitrary pedigrees were introduced by J. Ott in 1974 and were based on the powerful Elston - Stewart algorithm ( 1971 ) , which allowed likeli- hood calculations in pedigrees . The popularity of linkage analysis ...
Pagina 41
... SCORE Given a set of markers that are polymorphic and whose segregation has been observed in reference pedigrees ... score , Z ( 0 ) , is the statistical measure used to evaluate whether two loci are linked together . As defined below ...
... SCORE Given a set of markers that are polymorphic and whose segregation has been observed in reference pedigrees ... score , Z ( 0 ) , is the statistical measure used to evaluate whether two loci are linked together . As defined below ...
Pagina 42
... score and recombination - value criteria , but markers between the groups do not . It is recommended that the initial set- ting of the lod score be higher than the one calculated and that it be reduced in stages : Thus , reducing the score ...
... score and recombination - value criteria , but markers between the groups do not . It is recommended that the initial set- ting of the lod score be higher than the one calculated and that it be reduced in stages : Thus , reducing the score ...
Sommario
1 | |
16 | |
24 | |
33 | |
Genetic and Comparative Mapping in Mice | 71 |
MAPPING A GENE | 88 |
BACKCROSS ANALYSIS | 113 |
INTERCROSS ANALYSIS | 120 |
CLONING AND ANALYSIS OF THE FINAL DIFFERENCE PRODUCT | 252 |
Somatic Cell Genetics and Radiation Hybrid Mapping | 259 |
INTERSPECIFIC CELL HYBRIDS | 268 |
Fluorescence In Situ Hybridization | 303 |
FISH PROBES AND COUNTERSTAINS | 313 |
MAPPING APPLICATIONS FOR FISH | 327 |
FISH PROCEDURES | 333 |
PREPARATION OF DNA PROBES | 351 |
Identification and Analysis of DNA Polymorphisms | 135 |
IDENTIFICATION OF SNPs | 144 |
ANALYSIS OF SNPs | 153 |
IDENTIFICATION OF STRPs | 167 |
DNA Markers for Physical Mapping | 187 |
GENOMEWIDE STSS MARKERS FROM TOTAL GENOMIC LIBRARIES | 193 |
Purification of YAC DNA by PFGE AND Electroelution | 203 |
Representational Difference Analysis | 217 |
CHOOSING A RESTRICTION ENZYME FOR REPRESENTATION | 223 |
PROCEDURES FOR DIFFERENCE ENRICHMENT | 242 |
DENATURATION HYBRIDIZATION AND WASHING | 372 |
IMMUNOCYTOCHEMICAL DETECTION | 384 |
STAINING CHROMOSOMES AND CHROMOSOME BANDS | 391 |
MICROSCOPY | 405 |
Common Reagents | 415 |
Basic Procedures | 439 |
Safety Cautions | 465 |
Useful Facts | 473 |
Suppliers | 481 |
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20°C indefinitely adaptor agarose allele Amount to add amplified analyzing Appendix for Caution approximately backcross bands buffer cell lines chro chromosome clones Cold Spring Harbor Component and final containing DAPI described detection digested disease DNA samples DNA sequence dNTPs electrophoresis filter final concentration FITC fluorescence fluorochromes fragments frequency genetic map genomic DNA genotype H₂O heterozygotes heterozygous homozygous human chromosome human genome identified inbred Incubate intercross labeled laboratory large number ligation linkage analysis linkage map loci locus lod score markers medium methods mice mouse mutation Note oligonucleotide pair panel parental strain PCR assays PCR products pedigrees phenotype polymorphic positional cloning preparation primer probe progeny propidium iodide protocol radiation hybrid reagents recombination region repeat restriction enzyme RH mapping RI strains room temperature signals slides solution somatic cell hybrids step store at 20°C target tester tion trait tube volume µg/ml
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