An Introduction to Cell and Tissue CultureMary Imogene Bassett Hospital Tissue Culture Course (Cooperstown, N. Y.), Tissue Culture Course. Mary Imogene Bassett Hospital Burgess Publishing Company, 1955 - 123 pagine |
Dall'interno del libro
Risultati 1-3 di 11
Pagina 10
... Centrifuge at 3000 RPM for 20 minutes and dispense 1 ml aliquots of plasma to tubes . sert rubber stoppers and store the plasma at 4o C if it is to be used within a few weeks . Place in the -20 ° C freezer for longer periods of storage ...
... Centrifuge at 3000 RPM for 20 minutes and dispense 1 ml aliquots of plasma to tubes . sert rubber stoppers and store the plasma at 4o C if it is to be used within a few weeks . Place in the -20 ° C freezer for longer periods of storage ...
Pagina 42
... centrifuge tube . Stopper the tubes and centrifuge at 2000 RPM for 10 minutes . At the end of this time , a leukocyte layer should be floating at the plasma - albumin interface with 5 mm or more of clear albumin separating it from the ...
... centrifuge tube . Stopper the tubes and centrifuge at 2000 RPM for 10 minutes . At the end of this time , a leukocyte layer should be floating at the plasma - albumin interface with 5 mm or more of clear albumin separating it from the ...
Pagina 47
... Centrifuge suspension 1000 RPM for 5-10 minutes . Discard supernatant trypsin solution with a bent tip pipette . Resuspend cells evenly , using a 5 ml serologic pipette , in 7.5 ml human S - 50 , EE - 2 , BSS - 58 . Immediately dispense ...
... Centrifuge suspension 1000 RPM for 5-10 minutes . Discard supernatant trypsin solution with a bent tip pipette . Resuspend cells evenly , using a 5 ml serologic pipette , in 7.5 ml human S - 50 , EE - 2 , BSS - 58 . Immediately dispense ...
Sommario
Balanced Salt Solution Inorganic Requirements and pH control | 5 |
Plasma Serum Other Body Fluids and Derivatives | 9 |
Tissue Extracts and Derivatives | 11 |
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alcohol amino acids animal cells autoclaved balanced salt solution Biochem Biol blood body fluids Cancer Inst Carrel cell and tissue cell colonies cell cultures cell populations cell strains cell types cells in vitro cellular cent centrifuge chick embryo coverglass coverslip cytologic differentiation dilute drop EARLE embryo extract epithelial epithelium ethanol explants fibroblasts fibrocytes forceps glucose glycolysis HeLa J. H. Hanks laboratory lipids macrophages maintenance malignant mammalian metabolism methods migration minutes monocytes mouse NaHCO3 normal number of cells nutrient nutrition organ culture outgrowth oxidative Petri dish Physiol physiologic pipette plasma plasma clot poliomyelitis Porter flasks prepared Proc proliferation propagation proteins rates Rinse roller tube serum ultrafiltrate slide cultures sponge sterile stoppers strains of cells substrate surface synthetic tap water temperature test tube tion tissue cells tissue culture tissue fragments trypsin tumor tures ultrafiltrate utilization virus viruses vitro vivo watch glass