An Introduction to Cell and Tissue CultureMary Imogene Bassett Hospital Tissue Culture Course (Cooperstown, N. Y.), Tissue Culture Course. Mary Imogene Bassett Hospital Burgess Publishing Company, 1955 - 123 pagine |
Dall'interno del libro
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Pagina 7
... containing mammalian serum in the supernates ( 9 ) . Bonting and Jones ( 16 ) , how- ever , have reported the use of phosphate buffer at about seven times the usual concentra- tion in cultures maintained in high levels of chick embryo ...
... containing mammalian serum in the supernates ( 9 ) . Bonting and Jones ( 16 ) , how- ever , have reported the use of phosphate buffer at about seven times the usual concentra- tion in cultures maintained in high levels of chick embryo ...
Pagina 43
... containing 11 x 22 mm coverglasses . Each flask now contains the cells from 0.5 ml of blood . 7 . After 24 hours of incubation the monocytes will be attached to the glass . Shake the flask to dislodge unattached cells . Remove old ...
... containing 11 x 22 mm coverglasses . Each flask now contains the cells from 0.5 ml of blood . 7 . After 24 hours of incubation the monocytes will be attached to the glass . Shake the flask to dislodge unattached cells . Remove old ...
Pagina 45
... containing 1 ml of ox serum ultrafiltrate , add 2 ml of BSS and 0.3 ml of vaccinia virus ( from chicken chorioallantoic ( CA ) membranes ) . Place 20 drops of this mixture in each Porter flask . Stopper the flasks . 3 sterile 11 x 22 mm ...
... containing 1 ml of ox serum ultrafiltrate , add 2 ml of BSS and 0.3 ml of vaccinia virus ( from chicken chorioallantoic ( CA ) membranes ) . Place 20 drops of this mixture in each Porter flask . Stopper the flasks . 3 sterile 11 x 22 mm ...
Sommario
Balanced Salt Solution Inorganic Requirements and pH control | 5 |
Plasma Serum Other Body Fluids and Derivatives | 9 |
Tissue Extracts and Derivatives | 11 |
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Parole e frasi comuni
alcohol amino acids animal cells autoclaved balanced salt solution Biochem Biol blood body fluids Cancer Inst Carrel cell and tissue cell colonies cell cultures cell populations cell strains cell types cells in vitro cellular cent centrifuge chick embryo coverglass coverslip cytologic differentiation dilute drop EARLE embryo extract epithelial epithelium ethanol explants fibroblasts fibrocytes forceps glucose glycolysis HeLa J. H. Hanks laboratory lipids macrophages maintenance malignant mammalian metabolism methods migration minutes monocytes mouse NaHCO3 normal number of cells nutrient nutrition organ culture outgrowth oxidative Petri dish Physiol physiologic pipette plasma plasma clot poliomyelitis Porter flasks prepared Proc proliferation propagation proteins rates Rinse roller tube serum ultrafiltrate slide cultures sponge sterile stoppers strains of cells substrate surface synthetic tap water temperature test tube tion tissue cells tissue culture tissue fragments trypsin tumor tures ultrafiltrate utilization virus viruses vitro vivo watch glass