An Introduction to Cell and Tissue CultureMary Imogene Bassett Hospital Tissue Culture Course (Cooperstown, N. Y.), Tissue Culture Course. Mary Imogene Bassett Hospital Burgess Publishing Company, 1955 - 123 pagine |
Dall'interno del libro
Risultati 1-3 di 5
Pagina 3
... ethanol and allow to stand several minutes . Discard ethanol and drain pipettes with the fine tips up . Serological : 1. When collected , remove plugs ; assemble ( tips up ) in cylinder of C & M 1x until ready to wash . 2 . 3 . 4 . 5 ...
... ethanol and allow to stand several minutes . Discard ethanol and drain pipettes with the fine tips up . Serological : 1. When collected , remove plugs ; assemble ( tips up ) in cylinder of C & M 1x until ready to wash . 2 . 3 . 4 . 5 ...
Pagina 121
... ethanol ) , for 1-2 minutes . Control by periodic inspec- tion under the microscope . Transfer to ammonia water to ... ethanol 2 changes 6 . 95 % ethanol .. 2 changes 7. Absolute ethanol . 2 changes . 5 minutes . 5 minutes . 5 minutes ...
... ethanol ) , for 1-2 minutes . Control by periodic inspec- tion under the microscope . Transfer to ammonia water to ... ethanol 2 changes 6 . 95 % ethanol .. 2 changes 7. Absolute ethanol . 2 changes . 5 minutes . 5 minutes . 5 minutes ...
Pagina 122
... ethanol . For Differentiation : Stock Acid Alcohol 1 % HCl in 70 % ethyl alcohol . Stock Ammonia Water 5 drops of ammonium hydroxide per 100 ml tap water . For Dehydration , Clearing and Mounting : 80 % ethanol 95 % ethanol Absolute ethanol ...
... ethanol . For Differentiation : Stock Acid Alcohol 1 % HCl in 70 % ethyl alcohol . Stock Ammonia Water 5 drops of ammonium hydroxide per 100 ml tap water . For Dehydration , Clearing and Mounting : 80 % ethanol 95 % ethanol Absolute ethanol ...
Sommario
Balanced Salt Solution Inorganic Requirements and pH control | 5 |
Plasma Serum Other Body Fluids and Derivatives | 9 |
Tissue Extracts and Derivatives | 11 |
Copyright | |
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Parole e frasi comuni
alcohol amino acids animal cells autoclaved balanced salt solution Biochem Biol blood body fluids Cancer Inst Carrel cell and tissue cell colonies cell cultures cell populations cell strains cell types cells in vitro cellular cent centrifuge chick embryo coverglass coverslip cytologic differentiation dilute drop EARLE embryo extract epithelial epithelium ethanol explants fibroblasts fibrocytes forceps glucose glycolysis HeLa J. H. Hanks laboratory lipids macrophages maintenance malignant mammalian metabolism methods migration minutes monocytes mouse NaHCO3 normal number of cells nutrient nutrition organ culture outgrowth oxidative Petri dish Physiol physiologic pipette plasma plasma clot poliomyelitis Porter flasks prepared Proc proliferation propagation proteins rates Rinse roller tube serum ultrafiltrate slide cultures sponge sterile stoppers strains of cells substrate surface synthetic tap water temperature test tube tion tissue cells tissue culture tissue fragments trypsin tumor tures ultrafiltrate utilization virus viruses vitro vivo watch glass