An Introduction to Cell and Tissue CultureMary Imogene Bassett Hospital Tissue Culture Course (Cooperstown, N. Y.), Tissue Culture Course. Mary Imogene Bassett Hospital Burgess Publishing Company, 1955 - 123 pagine |
Dall'interno del libro
Risultati 1-3 di 33
Pagina 29
... explants than are required , pushing aside all inappropriate frag- ments . Rinse the selected explants with about 1 ml of thioglycollate medium and re- turn this fluid to the original tube for a sterility test . Further rinsing with BSS ...
... explants than are required , pushing aside all inappropriate frag- ments . Rinse the selected explants with about 1 ml of thioglycollate medium and re- turn this fluid to the original tube for a sterility test . Further rinsing with BSS ...
Pagina 35
... explants in 10 drops of EE - 12 or medium , and transfer to the flask . 3 . Mix plasma and EE - 12 , position the explants and allow the flask to lie on a flat surface until the plasma coagulates . 4 . Add 1 ml of fluid medium ( viz . S ...
... explants in 10 drops of EE - 12 or medium , and transfer to the flask . 3 . Mix plasma and EE - 12 , position the explants and allow the flask to lie on a flat surface until the plasma coagulates . 4 . Add 1 ml of fluid medium ( viz . S ...
Pagina 41
... explants trimmed in such fashion , that each explant shows a white , a gray and a red stratum . .ܢ 2 . Draw 20 ml ... explants at right angles to the surface of the stratified cells . Dissect the excess clear plasma from one side of ...
... explants trimmed in such fashion , that each explant shows a white , a gray and a red stratum . .ܢ 2 . Draw 20 ml ... explants at right angles to the surface of the stratified cells . Dissect the excess clear plasma from one side of ...
Sommario
Balanced Salt Solution Inorganic Requirements and pH control | 5 |
Plasma Serum Other Body Fluids and Derivatives | 9 |
Tissue Extracts and Derivatives | 11 |
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alcohol amino acids animal cells autoclaved balanced salt solution Biochem Biol blood body fluids Cancer Inst Carrel cell and tissue cell colonies cell cultures cell populations cell strains cell types cells in vitro cellular cent centrifuge chick embryo coverglass coverslip cytologic differentiation dilute drop EARLE embryo extract epithelial epithelium ethanol explants fibroblasts fibrocytes forceps glucose glycolysis HeLa J. H. Hanks laboratory lipids macrophages maintenance malignant mammalian metabolism methods migration minutes monocytes mouse NaHCO3 normal number of cells nutrient nutrition organ culture outgrowth oxidative Petri dish Physiol physiologic pipette plasma plasma clot poliomyelitis Porter flasks prepared Proc proliferation propagation proteins rates Rinse roller tube serum ultrafiltrate slide cultures sponge sterile stoppers strains of cells substrate surface synthetic tap water temperature test tube tion tissue cells tissue culture tissue fragments trypsin tumor tures ultrafiltrate utilization virus viruses vitro vivo watch glass