An Introduction to Cell and Tissue CultureMary Imogene Bassett Hospital Tissue Culture Course (Cooperstown, N. Y.), Tissue Culture Course. Mary Imogene Bassett Hospital Burgess Publishing Company, 1955 - 123 pagine |
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Pagina 43
... incubation the monocytes will be attached to the glass . Shake the flask to dislodge unattached cells . Remove old medium containing loose cells and re- place with fresh chicken serum , 30 % in BSS . After three days of incubation ...
... incubation the monocytes will be attached to the glass . Shake the flask to dislodge unattached cells . Remove old medium containing loose cells and re- place with fresh chicken serum , 30 % in BSS . After three days of incubation ...
Pagina 47
... incubate at 37 ° C. Three or 4 days later , add 0.3 ml ( 6 drops ) of human serum to each tube culture and each Porter flask and incubate for 1-3 additional days before proceeding to step B. 1 bent tip pipette 1 serol . pipette 5 ml 3 ...
... incubate at 37 ° C. Three or 4 days later , add 0.3 ml ( 6 drops ) of human serum to each tube culture and each Porter flask and incubate for 1-3 additional days before proceeding to step B. 1 bent tip pipette 1 serol . pipette 5 ml 3 ...
Pagina 55
... incubation , and by the composition and volume of medium . The suggestions tabulated below repre- sent rule of thumb schedules which have been found acceptable for routine maintenance of several cell types in the culture systems chosen ...
... incubation , and by the composition and volume of medium . The suggestions tabulated below repre- sent rule of thumb schedules which have been found acceptable for routine maintenance of several cell types in the culture systems chosen ...
Sommario
Balanced Salt Solution Inorganic Requirements and pH control | 5 |
Plasma Serum Other Body Fluids and Derivatives | 9 |
Tissue Extracts and Derivatives | 11 |
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alcohol amino acids animal cells autoclaved balanced salt solution Biochem Biol blood body fluids Cancer Inst Carrel cell and tissue cell colonies cell cultures cell populations cell strains cell types cells in vitro cellular cent centrifuge chick embryo coverglass coverslip cytologic differentiation dilute drop EARLE embryo extract epithelial epithelium ethanol explants fibroblasts fibrocytes forceps glucose glycolysis HeLa J. H. Hanks laboratory lipids macrophages maintenance malignant mammalian metabolism methods migration minutes monocytes mouse NaHCO3 normal number of cells nutrient nutrition organ culture outgrowth oxidative Petri dish Physiol physiologic pipette plasma plasma clot poliomyelitis Porter flasks prepared Proc proliferation propagation proteins rates Rinse roller tube serum ultrafiltrate slide cultures sponge sterile stoppers strains of cells substrate surface synthetic tap water temperature test tube tion tissue cells tissue culture tissue fragments trypsin tumor tures ultrafiltrate utilization virus viruses vitro vivo watch glass