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Pagina 6
Since evaporation of water occurs during every maneuver and especially from
thin preparations, it is wise to incorporate at least 10 per cent initial water excess
in all solutions prepared and to use screw caps or rubber stoppers as closures.
Since evaporation of water occurs during every maneuver and especially from
thin preparations, it is wise to incorporate at least 10 per cent initial water excess
in all solutions prepared and to use screw caps or rubber stoppers as closures.
Pagina 7
each is fitted with a paraffined cork stopper which has a fairly deep fissure from
the base to the level where the tube fits. When the stopper is raised a few
millimeters in the tube a port is opened for gas exchange and equilibration. As
the tubes ...
each is fitted with a paraffined cork stopper which has a fairly deep fissure from
the base to the level where the tube fits. When the stopper is raised a few
millimeters in the tube a port is opened for gas exchange and equilibration. As
the tubes ...
Pagina 10
Stopper firmly and mix by inversion. 4. Centrifuge at 3000 RPM for 20 minutes
and pipettes dispense 1 ml aliquots of plasma to tubes. In- 13 mm tubes sert
rubber stoppers and store the plasma at 00 rubber stoppers 4° C if it is to be used
...
Stopper firmly and mix by inversion. 4. Centrifuge at 3000 RPM for 20 minutes
and pipettes dispense 1 ml aliquots of plasma to tubes. In- 13 mm tubes sert
rubber stoppers and store the plasma at 00 rubber stoppers 4° C if it is to be used
...
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Indice
Chapter Page 1 The Laboratory Equipment and Supplies | 1 |
Glassware and Its Cleaning | 2 |
Balanced Salt Solution Inorganic Requirements and pH control | 5 |
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Parole e frasi comuni
albumin alcohol aliquots amino acids autoclaving balanced salt solution Biol blood body fluids Cancer Inst cell and tissue cell colonies cell cultures cell populations cell strains cell types cells in vitro cellular cent centrifuge chick embryo chicken plasma containing Coplin jar coverglass coverslip cultivation culture vessels cytologic dilute Discard EARLE embryo extract epithelial epithelium ethanol explants fibroblasts fibrocytes forceps glass glassware glucose glycolysis growth heparin incubation J. H. Hanks liquid medium living cells macrophages maintenance malignant mammalian metabolism methods migration monocytes mouse nutrient nutrition organ culture outgrowth Petri dish physiologic pipette plasma plasma clot poliomyelitis Porter flasks prepared Proc proliferation propagation proteins Remove Rinse roller tube Scherer serol serum ultrafiltrate slide cultures sponge sponge matrix stoppers substrate surface synthetic syringe tap water temperature test tube tion tissue culture tissue fragments transfer trypsin tube cultures tumor ultrafiltrate virus viruses vitro
Informazioni bibliografiche
| Titolo | An Introduction to Cell and Tissue Culture |
| Curatore | John h Hanks |
| Editore | Burgess, 1955 |
| Provenienza dell'originale | Università di Cornell |
| Digitalizzato | 7 feb 2009 |
| Lunghezza | 123 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |