An Introduction to Cell and Tissue CultureMary Imogene Bassett Hospital Tissue Culture Course (Cooperstown, N. Y.), Tissue Culture Course. Mary Imogene Bassett Hospital Burgess Publishing Company, 1955 - 123 pagine |
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Pagina 33
... TEST TUBE AND FLASK CULTURES J. Leighton Slide culture methods are convenient for the cytologic study of living cells and for fixed and stained preparations . However , since the volume of nutrient and the ... Test Tube and Flask Cultures.
... TEST TUBE AND FLASK CULTURES J. Leighton Slide culture methods are convenient for the cytologic study of living cells and for fixed and stained preparations . However , since the volume of nutrient and the ... Test Tube and Flask Cultures.
Pagina 45
... test tube 3 sterile Porter flasks 1 ml ox SUF in a 13x100 mm test tube 2 ml BSS with NaH 3 , 0 rubber stoppers 8 . Incubate at 37 ° C for 96 hours in a stationary position . Controls were not employed in this exercise . Other exercises ...
... test tube 3 sterile Porter flasks 1 ml ox SUF in a 13x100 mm test tube 2 ml BSS with NaH 3 , 0 rubber stoppers 8 . Incubate at 37 ° C for 96 hours in a stationary position . Controls were not employed in this exercise . Other exercises ...
Pagina 47
... test tubes Immediately slant cultures and incubate at 37 ° C. Three or 4 days later , add 0.3 ml ( 6 drops ) of human serum to each tube ... test tubes 17 00 stoppers 2 PF with c.g. 2 0 stoppers 1 box for slanting tubes 6 ml human serum 1 . 2 ...
... test tubes Immediately slant cultures and incubate at 37 ° C. Three or 4 days later , add 0.3 ml ( 6 drops ) of human serum to each tube ... test tubes 17 00 stoppers 2 PF with c.g. 2 0 stoppers 1 box for slanting tubes 6 ml human serum 1 . 2 ...
Sommario
Balanced Salt Solution Inorganic Requirements and pH control | 5 |
Plasma Serum Other Body Fluids and Derivatives | 9 |
Tissue Extracts and Derivatives | 11 |
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alcohol amino acids animal cells autoclaved balanced salt solution Biochem Biol blood body fluids Cancer Inst Carrel cell and tissue cell colonies cell cultures cell populations cell strains cell types cells in vitro cellular cent centrifuge chick embryo coverglass coverslip cytologic differentiation dilute drop EARLE embryo extract epithelial epithelium ethanol explants fibroblasts fibrocytes forceps glucose glycolysis HeLa J. H. Hanks laboratory lipids macrophages maintenance malignant mammalian metabolism methods migration minutes monocytes mouse NaHCO3 normal number of cells nutrient nutrition organ culture outgrowth oxidative Petri dish Physiol physiologic pipette plasma plasma clot poliomyelitis Porter flasks prepared Proc proliferation propagation proteins rates Rinse roller tube serum ultrafiltrate slide cultures sponge sterile stoppers strains of cells substrate surface synthetic tap water temperature test tube tion tissue cells tissue culture tissue fragments trypsin tumor tures ultrafiltrate utilization virus viruses vitro vivo watch glass