Laboratory Procedures in Clinical MicrobiologyJ.A. Washington Springer New York, 6 mag 1985 - 885 pagine Although there are a number of comprehensive books in clinical micro biology, there remains a need for a manual that can be used in the clinical laboratory to guide the daily performance of its work. Most of the existing publications provide detailed and precise information, for example, by which a microorganism can be characterized and identified beyond any doubt; however, the number of tests involved in this process exceeds the capabilities and resources of most clinical laboratories and are irrele vant for patient care. It is, therefore, necessary in any clinical laboratory to extract from reference manuals, textbooks, and journals those tests and procedures that are to be used to complete the daily workload as efficiently and accurately as possible. It is also essential in the clinical laboratory to determine, on the basis of the kind of specimen being exam ined, which microorganisms are clinically relevant and require isolation and identification and which should either be excluded selectively or simply regarded as indigenous flora and, therefore, not specifically identi fied. Cost and time limit a laboratory's resources, and priorities must be established for handling the workload. The procedures described in the second edition of this manual are those selected by our staff for use in the clinical laboratory on the basis of clinic~l relevance, accuracy, reproducibility, and efficiency. Alternative procedures, when considered equivalent on the basis of personal or pub lished experience, have been included where appropriate. |
Sommario
General Instructions for Specimen Collecting | 5 |
Specific Guidelines for Specimen Collection | 24 |
References | 67 |
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acid adenovirus aerobic anaerobic bacteria antibiotic antibody antigen antimicrobial antiserum assay bacteremia Bacteroides blood agar broth buffer Candida carbohydrate catalase cell cultures centrifuge Chlamydia Clin clinical specimens Clostridium cocci color concentration containing detect diagnosis Difco Laboratories differential dilution disease disk Dissolve Distilled water Distilled water Final echovirus Edwardsiella Enterobacteriaceae Escherichia coli examined fermentation Figure fluid fluorescence fungi gentamicin glucose gram-negative gram-negative bacilli gram-positive growth Haemophilus Haemophilus influenzae hyphae identification incubation infection influenzae inhibition inoculated inoculum Legionella medium method Microbiol microscopic morphology Motility mycobacteria Mycoplasma NaCl Negative test Neisseria nitrate organisms patients penicillin peptone plate pneumoniae Positive test prepared Procedure produce Pseudomonas reaction Reagents serological serum slant slide smear Sodium solution species stain Staphylococcus Sterilize at 121°C streptococci subculture susceptibility testing suspension swab Table tion tissue titers trachomatis urease urine usually vancomycin vial viral virus viruses water Final pH yeasts µg/ml
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