Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. BersonRolf Luft, Solomon A. Berson, Rosalyn Sussman Yalow, J. P. Accary Thieme, 1974 - 195 pagine |
Dall'interno del libro
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Pagina 8
... ACTH . Materials and Methods Preparation of Antibodies : Seven rabbits and 10 guinea pigs were immunized by repeated subcutaneous injections of 20 and 10 u , respectively , of ACTH - Z ( Organon ) emulsified in complete Freund's ...
... ACTH . Materials and Methods Preparation of Antibodies : Seven rabbits and 10 guinea pigs were immunized by repeated subcutaneous injections of 20 and 10 u , respectively , of ACTH - Z ( Organon ) emulsified in complete Freund's ...
Pagina 10
... ACTH 11-39 ACTH 1-24 ACTH 15-39 ACTH 120 100 80 Conventional Radioimmunoassay ( Antiserum R12x10000 ) 50 100 Bound Hormone ( 10M ) 150 Fig . 5 Standard curves obtained with intact antiserum ( R1 ) , its gamma globulin fraction and the ...
... ACTH 11-39 ACTH 1-24 ACTH 15-39 ACTH 120 100 80 Conventional Radioimmunoassay ( Antiserum R12x10000 ) 50 100 Bound Hormone ( 10M ) 150 Fig . 5 Standard curves obtained with intact antiserum ( R1 ) , its gamma globulin fraction and the ...
Pagina 69
... ACTH concentrations ( ng ACTH / 105 cells ) of rat pituitary cell suspensions in response to HSME and cortex extract ( CE ) 1 ) medium cells medium + cells control ( vehicle ) 1.53 ± 0.16 ICE 3.80 ± 0.43 0.125 HSME 4.73 ± 0.12 40.2 ...
... ACTH concentrations ( ng ACTH / 105 cells ) of rat pituitary cell suspensions in response to HSME and cortex extract ( CE ) 1 ) medium cells medium + cells control ( vehicle ) 1.53 ± 0.16 ICE 3.80 ± 0.43 0.125 HSME 4.73 ± 0.12 40.2 ...
Sommario
Methodology | 1 |
Applications to Physiology | 72 |
Tokuko Saito and Kazuo Shizume | 86 |
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Parole e frasi comuni
ACTH activity albumin amylase anti antibody antigen antisera antiserum arginine assay basal binding biological blood glucose bovine buffer C-peptide chymotrypsin chymotrypsinogen clin concentration cross-reactivity cyclic AMP diabetes dilution dose effect eluted Endocr Endocrinology enzymes erythropoietin estradiol estrogen extract fraction Freychet gastrin glucagon glucose tolerance GnRH gonadotropin granulated growth hormone HTSH human immunoassay immunoreactive increase incubation injection insulin levels insulin response islets isolated liver cells labelled LH and FSH LH response LH-RH LHRH luteinizing hormone measured membranes method mg/ml minutes ng/ml normal obesity oral pancreas pancreatic patients peak perifusion pituitary plasma insulin porcine pork preparations progesterone proinsulin proinsulin and insulin protein puberty purified rabbit radioactivity radioimmunoassay receptors releasing hormone S.A. Berson samples Sephadex serum sham feeding specific standard curve steroid stimulation subjects subunit Table theophylline thyroxine tion trypsin unlabeled