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Pagina 19
The rapid passage activity in a well - type automatic gamma counter through the
Dowex - 1 column was generally sufficient ... 2 cm x 85 cm was packed with
Sephadex 1930 1956 ) activities in extracts of bovine , porcine and rat G - 100 in
...
The rapid passage activity in a well - type automatic gamma counter through the
Dowex - 1 column was generally sufficient ... 2 cm x 85 cm was packed with
Sephadex 1930 1956 ) activities in extracts of bovine , porcine and rat G - 100 in
...
Pagina 36
0main peak and void volume regions were selected for pooling on the basis of
experiments ( such as that shown in Figure 1 ) in which the distribution of
biological activity in individual column fractions was related to the elution profile
of the ...
0main peak and void volume regions were selected for pooling on the basis of
experiments ( such as that shown in Figure 1 ) in which the distribution of
biological activity in individual column fractions was related to the elution profile
of the ...
Pagina 37
Human ) Total activity recovered as % of that loaded counts per second x103 le
% BOUND ( Sheep ) RADIOACTIVITY % BOUND ( 1 10 20 30 40 50 FRACTION
NUMBER 1 . 0 Fig . 3 Distribution of radioactivity of 135 1 human and 1311
sheep ...
Human ) Total activity recovered as % of that loaded counts per second x103 le
% BOUND ( Sheep ) RADIOACTIVITY % BOUND ( 1 10 20 30 40 50 FRACTION
NUMBER 1 . 0 Fig . 3 Distribution of radioactivity of 135 1 human and 1311
sheep ...
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ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography Clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocr Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreas patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin tubes University values weight Yalow