Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. BersonRolf Luft, Solomon A. Berson, Rosalyn Sussman Yalow, J. P. Accary Thieme, 1974 - 195 pagine |
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Pagina 3
... ADDED INSULIN M NO ADDED hGH ADDED INSULIN 0.2 ng / ml 3X103 . ADDED hGH 0.8 ng / ml ADDED INSULIN 1.0 ng / ml 2X103 . ADDED hGH 3.0 ng / ml 20 40 60 TUBE NUMBER 20 40 60 Fig . 3 Sephadex G100 gel filtration of antigen - antibody ...
... ADDED INSULIN M NO ADDED hGH ADDED INSULIN 0.2 ng / ml 3X103 . ADDED hGH 0.8 ng / ml ADDED INSULIN 1.0 ng / ml 2X103 . ADDED hGH 3.0 ng / ml 20 40 60 TUBE NUMBER 20 40 60 Fig . 3 Sephadex G100 gel filtration of antigen - antibody ...
Pagina 41
... added to adjust the pH to 6.1 . The solution was then subjected to anion exchange chromatography on a column ( d ... added 100 μl of diluted anti - proinsulin serum . After 20 h of incubation at 4 ° C , 100 μl of 125 I - Tyr - C ...
... added to adjust the pH to 6.1 . The solution was then subjected to anion exchange chromatography on a column ( d ... added 100 μl of diluted anti - proinsulin serum . After 20 h of incubation at 4 ° C , 100 μl of 125 I - Tyr - C ...
Pagina 81
... added to the incubation medium containing GTP ( 0.1 mM ) just before gassing . Glucagon was added 5 min later . The reaction was stopped by methanol at different times . Table 3 Effect of Trypsin on the cyclic AMP production of the ...
... added to the incubation medium containing GTP ( 0.1 mM ) just before gassing . Glucagon was added 5 min later . The reaction was stopped by methanol at different times . Table 3 Effect of Trypsin on the cyclic AMP production of the ...
Sommario
Methodology | 1 |
Applications to Physiology | 72 |
Tokuko Saito and Kazuo Shizume | 86 |
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Parole e frasi comuni
ACTH activity albumin amylase anti antibody antigen antisera antiserum arginine assay basal binding biological blood glucose bovine buffer C-peptide chymotrypsin chymotrypsinogen clin concentration cross-reactivity cyclic AMP diabetes dilution dose effect eluted Endocr Endocrinology enzymes erythropoietin estradiol estrogen extract fraction Freychet gastrin glucagon glucose tolerance GnRH gonadotropin granulated growth hormone HTSH human immunoassay immunoreactive increase incubation injection insulin levels insulin response islets isolated liver cells labelled LH and FSH LH response LH-RH LHRH luteinizing hormone measured membranes method mg/ml minutes ng/ml normal obesity oral pancreas pancreatic patients peak perifusion pituitary plasma insulin porcine pork preparations progesterone proinsulin proinsulin and insulin protein puberty purified rabbit radioactivity radioimmunoassay receptors releasing hormone S.A. Berson samples Sephadex serum sham feeding specific standard curve steroid stimulation subjects subunit Table theophylline thyroxine tion trypsin unlabeled