Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. BersonRolf Luft, Solomon A. Berson, Rosalyn Sussman Yalow, J. P. Accary Thieme, 1974 - 195 pagine |
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Pagina 6
... antibody . However this observation does not permit distinction between at least two of the possible explanations for hook effect - cooperativity or formation of non - precipitating antigen - antibody aggregates . Even as the ...
... antibody . However this observation does not permit distinction between at least two of the possible explanations for hook effect - cooperativity or formation of non - precipitating antigen - antibody aggregates . Even as the ...
Pagina 11
... antibody , inter- feres with separation of B and F seems unlikely , because the paradoxical phenomenon in radioimmu- noassay of ACTH was observed with three different methods of separation ; paperchromatoelectrophoresis , talc ...
... antibody , inter- feres with separation of B and F seems unlikely , because the paradoxical phenomenon in radioimmu- noassay of ACTH was observed with three different methods of separation ; paperchromatoelectrophoresis , talc ...
Pagina 16
... antibody Table 3 Approximate free energy , enthalpy and entropy of estradiol - antibody complexes Ko ( 109M - 1 ) A FO ( kcal / mole ) 4 ° C 26 ° C 37 ° C 5.38 1.65 0.906 2.01 0.818 0.489 interacting ligands . If a greater accessibility ...
... antibody Table 3 Approximate free energy , enthalpy and entropy of estradiol - antibody complexes Ko ( 109M - 1 ) A FO ( kcal / mole ) 4 ° C 26 ° C 37 ° C 5.38 1.65 0.906 2.01 0.818 0.489 interacting ligands . If a greater accessibility ...
Sommario
Methodology | 1 |
Applications to Physiology | 72 |
Tokuko Saito and Kazuo Shizume | 86 |
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Parole e frasi comuni
ACTH activity albumin amylase anti antibody antigen antisera antiserum arginine assay basal binding biological blood glucose bovine buffer C-peptide chymotrypsin chymotrypsinogen clin concentration cross-reactivity cyclic AMP diabetes dilution dose effect eluted Endocr Endocrinology enzymes erythropoietin estradiol estrogen extract fraction Freychet gastrin glucagon glucose tolerance GnRH gonadotropin granulated growth hormone HTSH human immunoassay immunoreactive increase incubation injection insulin levels insulin response islets isolated liver cells labelled LH and FSH LH response LH-RH LHRH luteinizing hormone measured membranes method mg/ml minutes ng/ml normal obesity oral pancreas pancreatic patients peak perifusion pituitary plasma insulin porcine pork preparations progesterone proinsulin proinsulin and insulin protein puberty purified rabbit radioactivity radioimmunoassay receptors releasing hormone S.A. Berson samples Sephadex serum sham feeding specific standard curve steroid stimulation subjects subunit Table theophylline thyroxine tion trypsin unlabeled