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Pagina 37
Human ) Total activity recovered as % of that loaded counts per second x103 le
% BOUND ( Sheep ) RADIOACTIVITY % BOUND ( 1 10 20 30 40 50 FRACTION
NUMBER 1 . 0 Fig . 3 Distribution of radioactivity of 135 1 human and 1311
sheep ...
Human ) Total activity recovered as % of that loaded counts per second x103 le
% BOUND ( Sheep ) RADIOACTIVITY % BOUND ( 1 10 20 30 40 50 FRACTION
NUMBER 1 . 0 Fig . 3 Distribution of radioactivity of 135 1 human and 1311
sheep ...
Pagina 75
4 ) , the amounts of glucagon bound ( 0 . 18 + 0 . 01 Competitive inhibition of the
125 1 - hormone binding picomole / 106 cells , n = 4 ) and insulin bound ( 0 . 13
by unlabeled homologous hormone . Unlabeled insulin + 0 . 01 picomole / 106 ...
4 ) , the amounts of glucagon bound ( 0 . 18 + 0 . 01 Competitive inhibition of the
125 1 - hormone binding picomole / 106 cells , n = 4 ) and insulin bound ( 0 . 13
by unlabeled homologous hormone . Unlabeled insulin + 0 . 01 picomole / 106 ...
Pagina 89
x 103 COUNT 1251 - HGH BOUND TO RAT LIVER - CELL MEMBRANES 1251 -
HGH BOUND 1 . 0 MEMBRANES mg DRY WEIGHT 0 30 60 MINUTES 90 120
Fig . 2 left Binding of 125 I - hGH to liver cell membranes as a function of the dry ...
x 103 COUNT 1251 - HGH BOUND TO RAT LIVER - CELL MEMBRANES 1251 -
HGH BOUND 1 . 0 MEMBRANES mg DRY WEIGHT 0 30 60 MINUTES 90 120
Fig . 2 left Binding of 125 I - hGH to liver cell membranes as a function of the dry ...
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ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography Clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocr Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreas patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin tubes University values weight Yalow
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Informazioni bibliografiche
| Titolo | Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. Berson Volume 5 di Hormone and metabolic research / Supplement series Edizione 5 di Hormone and metabolic research: Supplement series, ISSN 0170-5903 Volume 5 di Suppl. series, Hormone and metabolic research |
| Curatori | Solomon A. Berson, Rolf Luft, Rosalyn Sussman Yalow, J. P. Accary |
| Editore | Thieme, 1974 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 1 ago 2008 |
| ISBN | 3135147010, 9783135147017 |
| Lunghezza | 195 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |