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Pagina 13
01 M phosphate buffer , pH 6 , at 25°C for 6 hrs . methods , such as equilibrium
dialysis and gel equi - The antibody - Sepharose derivative was washed three
libration . However , a method based on the use of times with 0 , 025 M tris -
EDTA ...
01 M phosphate buffer , pH 6 , at 25°C for 6 hrs . methods , such as equilibrium
dialysis and gel equi - The antibody - Sepharose derivative was washed three
libration . However , a method based on the use of times with 0 , 025 M tris -
EDTA ...
Pagina 62
05 PO4 buffer , pH 7 . 4 , in the presence of 12 . 5 AG ) by intravenous injection
into an ante - cubital My chloramine T ( in 10 ml of this same buffer ) ; for vein . 30
seconds . The reaction was stopped by the addiBlood samples were drawn from
...
05 PO4 buffer , pH 7 . 4 , in the presence of 12 . 5 AG ) by intravenous injection
into an ante - cubital My chloramine T ( in 10 ml of this same buffer ) ; for vein . 30
seconds . The reaction was stopped by the addiBlood samples were drawn from
...
Pagina 87
000 rpm for 30 seconds , three times suc membranes at 4°C with a Nihon Seiki
homogenizer cessively . The homogenate was centrifuged at 600 at 10 . 000 rpm
in Krebs - Ringer bicarbonate buffer xg for 10 minutes . The supernatant was ...
000 rpm for 30 seconds , three times suc membranes at 4°C with a Nihon Seiki
homogenizer cessively . The homogenate was centrifuged at 600 at 10 . 000 rpm
in Krebs - Ringer bicarbonate buffer xg for 10 minutes . The supernatant was ...
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ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography Clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocr Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreas patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin tubes University values weight Yalow
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Informazioni bibliografiche
| Titolo | Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. Berson Volume 5 di Hormone and metabolic research / Supplement series Edizione 5 di Hormone and metabolic research: Supplement series, ISSN 0170-5903 Volume 5 di Suppl. series, Hormone and metabolic research |
| Curatori | Solomon A. Berson, Rolf Luft, Rosalyn Sussman Yalow, J. P. Accary |
| Editore | Thieme, 1974 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 1 ago 2008 |
| ISBN | 3135147010, 9783135147017 |
| Lunghezza | 195 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |