Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. BersonRolf Luft, Solomon A. Berson, Rosalyn Sussman Yalow, J. P. Accary Thieme, 1974 - 195 pagine |
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Pagina 80
... cyclic AMP and of theophylline in the radioimmunoassay of the cyclic AMP content of isolated liver cells . The effects of the substances were measured with 3 different rabbit immunsera : 302/5 and 31/4 at 1 : 40.000 301/8 at 1 : 60,000 ...
... cyclic AMP and of theophylline in the radioimmunoassay of the cyclic AMP content of isolated liver cells . The effects of the substances were measured with 3 different rabbit immunsera : 302/5 and 31/4 at 1 : 40.000 301/8 at 1 : 60,000 ...
Pagina 83
... Cyclic AMP , picomoles / 106 liver cells Cyclic AMP produced , percent of maximum 100r cyclic AMP production Glucagon 12 AM to Quantity of cyclic AMP , picomoles per 10 ° LC 200 C 37.3 1.7 300 C 58.6 ± 2.1 370 C 60.5 + 1.9 1251 ...
... Cyclic AMP , picomoles / 106 liver cells Cyclic AMP produced , percent of maximum 100r cyclic AMP production Glucagon 12 AM to Quantity of cyclic AMP , picomoles per 10 ° LC 200 C 37.3 1.7 300 C 58.6 ± 2.1 370 C 60.5 + 1.9 1251 ...
Pagina 84
... cyclic AMP production ( picomoles ) 100 % ( percent of maximum ) 100 cyclic AMP production , picomoles 50- ю refed rat no insulin . with insulin fasted rat no insulin with insulin 0.1 ( picomoles ) 93 Fig . 4 Effect of glucagon ...
... cyclic AMP production ( picomoles ) 100 % ( percent of maximum ) 100 cyclic AMP production , picomoles 50- ю refed rat no insulin . with insulin fasted rat no insulin with insulin 0.1 ( picomoles ) 93 Fig . 4 Effect of glucagon ...
Sommario
Methodology | 1 |
Applications to Physiology | 72 |
Tokuko Saito and Kazuo Shizume | 86 |
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ACTH activity albumin amylase anti antibody antigen antisera antiserum arginine assay basal binding biological blood glucose bovine buffer C-peptide chymotrypsin chymotrypsinogen clin concentration cross-reactivity cyclic AMP diabetes dilution dose effect eluted Endocr Endocrinology enzymes erythropoietin estradiol estrogen extract fraction Freychet gastrin glucagon glucose tolerance GnRH gonadotropin granulated growth hormone HTSH human immunoassay immunoreactive increase incubation injection insulin levels insulin response islets isolated liver cells labelled LH and FSH LH response LH-RH LHRH luteinizing hormone measured membranes method mg/ml minutes ng/ml normal obesity oral pancreas pancreatic patients peak perifusion pituitary plasma insulin porcine pork preparations progesterone proinsulin proinsulin and insulin protein puberty purified rabbit radioactivity radioimmunoassay receptors releasing hormone S.A. Berson samples Sephadex serum sham feeding specific standard curve steroid stimulation subjects subunit Table theophylline thyroxine tion trypsin unlabeled