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Pagina 3
10 , 000 DILUTION ANTI - AGH GUINEA PIG 4 - 5 140 , 000 DILUTION ANTI -
ACTH GUINEA PIGS 1 : 1000 DILUTION SEPHADEX G100 GEL FILTRATION OF
ANTIGEN - ANTIBODY INCUBATION MIXTURES ANTI - INSULIN ANTI - NGH ...
10 , 000 DILUTION ANTI - AGH GUINEA PIG 4 - 5 140 , 000 DILUTION ANTI -
ACTH GUINEA PIGS 1 : 1000 DILUTION SEPHADEX G100 GEL FILTRATION OF
ANTIGEN - ANTIBODY INCUBATION MIXTURES ANTI - INSULIN ANTI - NGH ...
Pagina 4
Table 1 Count rate distribution following ultracentrifugation ( 40 . 000 RPM - 7
hours - 4°C ) Insulin Guinea pig 7 ; 1 : 1000 dilution ; 1 day incubation 1 ml No
centrifugation Centrifugation Layers Control Control Tracer St . Curve : 0 . 2 mu /
ml St ...
Table 1 Count rate distribution following ultracentrifugation ( 40 . 000 RPM - 7
hours - 4°C ) Insulin Guinea pig 7 ; 1 : 1000 dilution ; 1 day incubation 1 ml No
centrifugation Centrifugation Layers Control Control Tracer St . Curve : 0 . 2 mu /
ml St ...
Pagina 5
0 INSULIN CONCENTRATION - ng / ml serum at the same dilution . The peak B /
F ratio was Fig . 7 Standard curves for the radioimmunoassay of insulin 2 . 65
using the unfractionated antiserum ( Fig . 1 left ) : using Fab fragments at ...
0 INSULIN CONCENTRATION - ng / ml serum at the same dilution . The peak B /
F ratio was Fig . 7 Standard curves for the radioimmunoassay of insulin 2 . 65
using the unfractionated antiserum ( Fig . 1 left ) : using Fab fragments at ...
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ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography Clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocr Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreas patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin tubes University values weight Yalow
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Informazioni bibliografiche
| Titolo | Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. Berson Volume 5 di Hormone and metabolic research / Supplement series Edizione 5 di Hormone and metabolic research: Supplement series, ISSN 0170-5903 Volume 5 di Suppl. series, Hormone and metabolic research |
| Curatori | Solomon A. Berson, Rolf Luft, Rosalyn Sussman Yalow, J. P. Accary |
| Editore | Thieme, 1974 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 1 ago 2008 |
| ISBN | 3135147010, 9783135147017 |
| Lunghezza | 195 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |