Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. BersonRolf Luft, Solomon A. Berson, Rosalyn Sussman Yalow, J. P. Accary Thieme, 1974 - 195 pagine |
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Pagina 76
... experiments for insulin and 4 repeated experiments for glucagon . The insert to the right represents the amounts of hormone ( glucagon : open circles , dotted line ; insulin : closed circles , solid line ) bound at low concen- tration ...
... experiments for insulin and 4 repeated experiments for glucagon . The insert to the right represents the amounts of hormone ( glucagon : open circles , dotted line ; insulin : closed circles , solid line ) bound at low concen- tration ...
Pagina 93
... experiments , peak levels of plasma insulin were reached 2-4 min after initiation of sham feeding . Although the animals in these experiments were still eating , plasma insulin levels declined during the latter period of stimulation ...
... experiments , peak levels of plasma insulin were reached 2-4 min after initiation of sham feeding . Although the animals in these experiments were still eating , plasma insulin levels declined during the latter period of stimulation ...
Pagina 98
... experiments . For the 60 min . pulse period , batches of islets ( 30-40 ) were incubated in 500 μl KRBB supplemented with glucose ( 0.6 or 3.0 mg / ml ) , bovine serum albumin ( 10 mg / ml ) , 3H - leucine or 3H - tyrosine ( 20 pCi / ml ) ...
... experiments . For the 60 min . pulse period , batches of islets ( 30-40 ) were incubated in 500 μl KRBB supplemented with glucose ( 0.6 or 3.0 mg / ml ) , bovine serum albumin ( 10 mg / ml ) , 3H - leucine or 3H - tyrosine ( 20 pCi / ml ) ...
Sommario
Methodology | 1 |
Applications to Physiology | 72 |
Tokuko Saito and Kazuo Shizume | 86 |
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ACTH activity albumin amylase anti antibody antigen antisera antiserum arginine assay basal binding biological blood glucose bovine buffer C-peptide chymotrypsin chymotrypsinogen clin concentration cross-reactivity cyclic AMP diabetes dilution dose effect eluted Endocr Endocrinology enzymes erythropoietin estradiol estrogen extract fraction Freychet gastrin glucagon glucose tolerance GnRH gonadotropin granulated growth hormone HTSH human immunoassay immunoreactive increase incubation injection insulin levels insulin response islets isolated liver cells labelled LH and FSH LH response LH-RH LHRH luteinizing hormone measured membranes method mg/ml minutes ng/ml normal obesity oral pancreas pancreatic patients peak perifusion pituitary plasma insulin porcine pork preparations progesterone proinsulin proinsulin and insulin protein puberty purified rabbit radioactivity radioimmunoassay receptors releasing hormone S.A. Berson samples Sephadex serum sham feeding specific standard curve steroid stimulation subjects subunit Table theophylline thyroxine tion trypsin unlabeled