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Pagina 74
4x10R / ml incubation medium ( time 0 ) at the indicated temperatures , in the
absence and in the presence of unlabeled homologous hormone at 10 uM to
determine the non specific binding ( see Methods ) . At the indicated times , cell -
bound ...
4x10R / ml incubation medium ( time 0 ) at the indicated temperatures , in the
absence and in the presence of unlabeled homologous hormone at 10 uM to
determine the non specific binding ( see Methods ) . At the indicated times , cell -
bound ...
Pagina 80
000 301 / 8 at 1 : 60 , 000 final using the radioimmunoassay of cyclic AMP
indicated in the text . The initial binding of the radioiodinated derivative of cyclic
AMP ( " 251 - Suc AMP - Tyr - OMe ) is the percentage of the total radioactivity
bound in ...
000 301 / 8 at 1 : 60 , 000 final using the radioimmunoassay of cyclic AMP
indicated in the text . The initial binding of the radioiodinated derivative of cyclic
AMP ( " 251 - Suc AMP - Tyr - OMe ) is the percentage of the total radioactivity
bound in ...
Pagina 83
After one minute gassing , the incubation was performed at 20°C ( O ) , 30°C ( 0 )
or 37°C ( - ) ; glucagon ( 1 . 2 nM ) was added after 10 min . a ) In the cyclic AMP
measurement , indicated to the left , the reaction was terminated by methanol .
After one minute gassing , the incubation was performed at 20°C ( O ) , 30°C ( 0 )
or 37°C ( - ) ; glucagon ( 1 . 2 nM ) was added after 10 min . a ) In the cyclic AMP
measurement , indicated to the left , the reaction was terminated by methanol .
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ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography Clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocr Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreas patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin tubes University values weight Yalow