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Pagina 99
100 ul Incubation of granulated islets with ' H - leucine for a aliquots were
incubated at 37°C for 20 min . in 60 min . pulse period in 0 . 6 mg / ml glucose
resulted 900 ul 0 . 05 M tris - HCl ( pH 7 . 5 ) / 20 mM CaCl2 with rith in the
recovery of low ...
100 ul Incubation of granulated islets with ' H - leucine for a aliquots were
incubated at 37°C for 20 min . in 60 min . pulse period in 0 . 6 mg / ml glucose
resulted 900 ul 0 . 05 M tris - HCl ( pH 7 . 5 ) / 20 mM CaCl2 with rith in the
recovery of low ...
Pagina 100
Table 2 Percentage of newly synthesised proinsulin in islets and media from
pulse / chase experiments ( mean + SEM ) Glucose conc . ( mg / ml ) Percentage
of proinsulin in islets in media pulse chase pulse chase chase Fig . 3 Granulated
...
Table 2 Percentage of newly synthesised proinsulin in islets and media from
pulse / chase experiments ( mean + SEM ) Glucose conc . ( mg / ml ) Percentage
of proinsulin in islets in media pulse chase pulse chase chase Fig . 3 Granulated
...
Pagina 101
islets , pulse 60 islets , chose 120 islets , putse 60 ' SP 70 % islets , chose 120 PI
83 % PI 50 % Pl 70 % E - OL * wdp HE ... The G - 50 radioactivity patterns for the
pulse islet and media samples are shown on the left while those for the chase ...
islets , pulse 60 islets , chose 120 islets , putse 60 ' SP 70 % islets , chose 120 PI
83 % PI 50 % Pl 70 % E - OL * wdp HE ... The G - 50 radioactivity patterns for the
pulse islet and media samples are shown on the left while those for the chase ...
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Parole e frasi comuni
ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography Clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocr Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreas patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin tubes University values weight Yalow
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Informazioni bibliografiche
| Titolo | Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. Berson Volume 5 di Hormone and metabolic research / Supplement series Edizione 5 di Hormone and metabolic research: Supplement series, ISSN 0170-5903 Volume 5 di Suppl. series, Hormone and metabolic research |
| Curatori | Solomon A. Berson, Rolf Luft, Rosalyn Sussman Yalow, J. P. Accary |
| Editore | Thieme, 1974 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 1 ago 2008 |
| ISBN | 3135147010, 9783135147017 |
| Lunghezza | 195 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |