Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. BersonRolf Luft, Solomon A. Berson, Rosalyn Sussman Yalow, J. P. Accary Thieme, 1974 - 195 pagine |
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Pagina 87
... membranes For some hGH binding studies rat liver membranes of the 40.000 x g pellet were solubilized . The pellet was suspended in 0.05 M Tris HCl buffer containing 0.2 % W / v ) Triton X - 100 , incubated for 30 minutes at 27 ° C , and ...
... membranes For some hGH binding studies rat liver membranes of the 40.000 x g pellet were solubilized . The pellet was suspended in 0.05 M Tris HCl buffer containing 0.2 % W / v ) Triton X - 100 , incubated for 30 minutes at 27 ° C , and ...
Pagina 89
... membranes as a function of the dry weight of membrane in the absence ( 0–0 ) and in the presence ( • • ) of 10 μg hGH . Right Time course of binding of 125 - I - hGH to 2 mg ( dry weight of membranes at 24 ° C ( • and at 37 ° C ( oo ) ...
... membranes as a function of the dry weight of membrane in the absence ( 0–0 ) and in the presence ( • • ) of 10 μg hGH . Right Time course of binding of 125 - I - hGH to 2 mg ( dry weight of membranes at 24 ° C ( • and at 37 ° C ( oo ) ...
Pagina 90
... membranes in suspension rather than soluble membrane receptors were used for radio- receptor assay purposes . Ultracentrifugation was em- ployed for separation of bound and free labelled hormone . Although we have demonstrated specific ...
... membranes in suspension rather than soluble membrane receptors were used for radio- receptor assay purposes . Ultracentrifugation was em- ployed for separation of bound and free labelled hormone . Although we have demonstrated specific ...
Sommario
Methodology | 1 |
Applications to Physiology | 72 |
Tokuko Saito and Kazuo Shizume | 86 |
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Parole e frasi comuni
ACTH activity albumin amylase anti antibody antigen antisera antiserum arginine assay basal binding biological blood glucose bovine buffer C-peptide chymotrypsin chymotrypsinogen clin concentration cross-reactivity cyclic AMP diabetes dilution dose effect eluted Endocr Endocrinology enzymes erythropoietin estradiol estrogen extract fraction Freychet gastrin glucagon glucose tolerance GnRH gonadotropin granulated growth hormone HTSH human immunoassay immunoreactive increase incubation injection insulin levels insulin response islets isolated liver cells labelled LH and FSH LH response LH-RH LHRH luteinizing hormone measured membranes method mg/ml minutes ng/ml normal obesity oral pancreas pancreatic patients peak perifusion pituitary plasma insulin porcine pork preparations progesterone proinsulin proinsulin and insulin protein puberty purified rabbit radioactivity radioimmunoassay receptors releasing hormone S.A. Berson samples Sephadex serum sham feeding specific standard curve steroid stimulation subjects subunit Table theophylline thyroxine tion trypsin unlabeled