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Pagina 10
5 Standard curves obtained with intact antiserum ( R1 ) , Fig . 6 Reactivity of a ' •
24 - ACTH , its N - terminal fragment its gamma globulin fraction and the first
piece ( Fab fractions ) ( al - 34 - ACTH ) and its C - terminal fragments ( an ' s - 3 ...
5 Standard curves obtained with intact antiserum ( R1 ) , Fig . 6 Reactivity of a ' •
24 - ACTH , its N - terminal fragment its gamma globulin fraction and the first
piece ( Fab fractions ) ( al - 34 - ACTH ) and its C - terminal fragments ( an ' s - 3 ...
Pagina 32
... A. Berson Solomon A. Berson, Rolf Luft, J. P. Accary. Table 1 Binding capacity
expressed in B / T ratio ( % ) of the antisera obtained by the classical
immunization procedure Dilutions 100 500 1000 5000 Rabbit NO Bleeding NO iš
zó 1 . 6 2 .
... A. Berson Solomon A. Berson, Rolf Luft, J. P. Accary. Table 1 Binding capacity
expressed in B / T ratio ( % ) of the antisera obtained by the classical
immunization procedure Dilutions 100 500 1000 5000 Rabbit NO Bleeding NO iš
zó 1 . 6 2 .
Pagina 192
The day of the maximum concentration of plasma LH was arbitrarily designated
as day 0 . m indicates the onset of menstruation . phosphate buffer were
superimposable with those G . E . , a first specimen , obtained when he was on
obtained ...
The day of the maximum concentration of plasma LH was arbitrarily designated
as day 0 . m indicates the onset of menstruation . phosphate buffer were
superimposable with those G . E . , a first specimen , obtained when he was on
obtained ...
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ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography Clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocr Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreas patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin tubes University values weight Yalow