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Pagina 99
The second peak contained roughly proinsulin eluted from 39 - 43 ml while
porcine insulin 80 % of the recovered radioactivity in both ... Cycloheximide
inhibited incorporaTrypsinisation of the G - 50 proinsulin peak tion into these
peaks ( Fig .
The second peak contained roughly proinsulin eluted from 39 - 43 ml while
porcine insulin 80 % of the recovered radioactivity in both ... Cycloheximide
inhibited incorporaTrypsinisation of the G - 50 proinsulin peak tion into these
peaks ( Fig .
Pagina 110
Gastrin ( ng . ml - 1 ) . 04 M - - - - - - - - NH 4 HCO3 gradient - - - - - - - 3M
PANCREAS peak A Table 2 Elution from AE 41 aminoethylcellulose Amount of
pancreatic tissue : 2 . 400 g . ( A ) peak A - 1 50 ng = 22 . 2 pg / g tissue A - 2 41
ng = 17 .
Gastrin ( ng . ml - 1 ) . 04 M - - - - - - - - NH 4 HCO3 gradient - - - - - - - 3M
PANCREAS peak A Table 2 Elution from AE 41 aminoethylcellulose Amount of
pancreatic tissue : 2 . 400 g . ( A ) peak A - 1 50 ng = 22 . 2 pg / g tissue A - 2 41
ng = 17 .
Pagina 165
Table 4 Fasting and peak concentrations of plasma glucagon and insulin and
blood glucose during arginine stimulation test in 4 children with isolated HGH
deficiency tested before and during long term HGH treatment Patient Glucagon
Pg / ml ...
Table 4 Fasting and peak concentrations of plasma glucagon and insulin and
blood glucose during arginine stimulation test in 4 children with isolated HGH
deficiency tested before and during long term HGH treatment Patient Glucagon
Pg / ml ...
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ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography Clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocr Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreas patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin tubes University values weight Yalow