Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. BersonRolf Luft, Solomon A. Berson, Rosalyn Sussman Yalow, J. P. Accary Thieme, 1974 - 195 pagine |
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Pagina 99
... peak following the void volume peak was subjected to limited tryptic hydro- lysis . These G - 50 peak fractions were lyophilised and reconstituted in 1 000 μl 0.01 N HCl . 100 μl aliquots were incubated at 37 ° C for 20 min . in 900 μl ...
... peak following the void volume peak was subjected to limited tryptic hydro- lysis . These G - 50 peak fractions were lyophilised and reconstituted in 1 000 μl 0.01 N HCl . 100 μl aliquots were incubated at 37 ° C for 20 min . in 900 μl ...
Pagina 110
... peak A A 2 2.0 ( B ) peak B - 1 B - 2 110 ng = 420 ng 45 . pg / g tissue 1.6 175 . pg / g tissue 1.2 .8 Note that the amount of gastrin recovered increases after purification on AE 41 We note that the elution of antrum extract shows ...
... peak A A 2 2.0 ( B ) peak B - 1 B - 2 110 ng = 420 ng 45 . pg / g tissue 1.6 175 . pg / g tissue 1.2 .8 Note that the amount of gastrin recovered increases after purification on AE 41 We note that the elution of antrum extract shows ...
Pagina 165
... Peak Insulin Fast μU / ml Peak Glucose Fast mg / 10 ml Peak B D B BD BD B D D.Z I.E. E.N. B.B. Mean B = MMMF 4 99 70 220 97 11 7 12 13 75 88 69 102 9 147 70 312 288 11 17 25 23 87 64 74 79 11 75 95 311 144 1 10 10 12 151 91 309 352 3 11 ...
... Peak Insulin Fast μU / ml Peak Glucose Fast mg / 10 ml Peak B D B BD BD B D D.Z I.E. E.N. B.B. Mean B = MMMF 4 99 70 220 97 11 7 12 13 75 88 69 102 9 147 70 312 288 11 17 25 23 87 64 74 79 11 75 95 311 144 1 10 10 12 151 91 309 352 3 11 ...
Sommario
Methodology | 1 |
Applications to Physiology | 72 |
Tokuko Saito and Kazuo Shizume | 86 |
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Parole e frasi comuni
ACTH activity albumin amylase anti antibody antigen antisera antiserum arginine assay basal binding biological blood glucose bovine buffer C-peptide chymotrypsin chymotrypsinogen clin concentration cross-reactivity cyclic AMP diabetes dilution dose effect eluted Endocr Endocrinology enzymes erythropoietin estradiol estrogen extract fraction Freychet gastrin glucagon glucose tolerance GnRH gonadotropin granulated growth hormone HTSH human immunoassay immunoreactive increase incubation injection insulin levels insulin response islets isolated liver cells labelled LH and FSH LH response LH-RH LHRH luteinizing hormone measured membranes method mg/ml minutes ng/ml normal obesity oral pancreas pancreatic patients peak perifusion pituitary plasma insulin porcine pork preparations progesterone proinsulin proinsulin and insulin protein puberty purified rabbit radioactivity radioimmunoassay receptors releasing hormone S.A. Berson samples Sephadex serum sham feeding specific standard curve steroid stimulation subjects subunit Table theophylline thyroxine tion trypsin unlabeled