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Pagina 83
2AM 30°C Fig . 3 Comparative effects of varying temperature on the glucagon
interaction with the rat isolated liver cells . On the upper part : Time course for
cycic AMP accumulation and binding of glucagon was performed in the same
medium ...
2AM 30°C Fig . 3 Comparative effects of varying temperature on the glucagon
interaction with the rat isolated liver cells . On the upper part : Time course for
cycic AMP accumulation and binding of glucagon was performed in the same
medium ...
Pagina 92
In plasma was frozen and kept at - 20° until determinaassays unknown plasmas
were generally assayed at a tion of the insulin was performed by radioimmuno1 :
25 dilution , the final volume of the incubation assay . mixture being 2 . 5 ml .
In plasma was frozen and kept at - 20° until determinaassays unknown plasmas
were generally assayed at a tion of the insulin was performed by radioimmuno1 :
25 dilution , the final volume of the incubation assay . mixture being 2 . 5 ml .
Pagina 167
The experiments with lactate administration were performed on the female group
with body weight of 50 - 62 kg . The procedure had been carefully explained to all
the subjects to minimize the influence of emo . tional stress . For simulation of ...
The experiments with lactate administration were performed on the female group
with body weight of 50 - 62 kg . The procedure had been carefully explained to all
the subjects to minimize the influence of emo . tional stress . For simulation of ...
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ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography Clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocr Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreas patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin tubes University values weight Yalow