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Pagina 98
In one series Material and Methods of experiments the pulse period was
extended to Materials . L - 14 , 5 - 3H ] – leucine ( 36 Ci / mmol ) and 120 min .
and cycloheximide ( 250 ug / ml ) was added L - [ 3 , 5 - ' H ] - tyrosine ( 36 Ci /
mmol ) were ...
In one series Material and Methods of experiments the pulse period was
extended to Materials . L - 14 , 5 - 3H ] – leucine ( 36 Ci / mmol ) and 120 min .
and cycloheximide ( 250 ug / ml ) was added L - [ 3 , 5 - ' H ] - tyrosine ( 36 Ci /
mmol ) were ...
Pagina 100
No significant release of radioactive proinsulin or insulin was detected in the
media during this 60 min . pulse period . After the 120 min . chase period ( 3 . 0
mg / ml glucose ) some radioactivity was still recovered in the islets ( 55 + 3 % in
...
No significant release of radioactive proinsulin or insulin was detected in the
media during this 60 min . pulse period . After the 120 min . chase period ( 3 . 0
mg / ml glucose ) some radioactivity was still recovered in the islets ( 55 + 3 % in
...
Pagina 115
Table 3 shows the time course of the changes in k - values in those high and low
insulin responders that developed decreased glucose tolerance during the
observation period . It is notable that in the majority of subjects - - even in those
with ...
Table 3 shows the time course of the changes in k - values in those high and low
insulin responders that developed decreased glucose tolerance during the
observation period . It is notable that in the majority of subjects - - even in those
with ...
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ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreatic patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin University values weight Yalow