Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. BersonRolf Luft, Solomon A. Berson, Rosalyn Sussman Yalow, J. P. Accary Thieme, 1974 - 195 pagine |
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Risultati 1-3 di 37
Pagina 97
... period ( 0.6 or 3.0 mg / ml glucose ) . However , when granulated and degranulated islets were pulsed with 3H - leucine for 60 min . with 3.0 mg / ml glucose , radioactive pro- insulin represented 80 ± 3 % and 83 ± 2 % of the recovered ...
... period ( 0.6 or 3.0 mg / ml glucose ) . However , when granulated and degranulated islets were pulsed with 3H - leucine for 60 min . with 3.0 mg / ml glucose , radioactive pro- insulin represented 80 ± 3 % and 83 ± 2 % of the recovered ...
Pagina 98
... periods . Incubations were performed in a shaking water bath at 37 ° C . After the pulse period , the medium was withdrawn and the islets were washed twice with buffer . Islets from half of the incubation vials were homogenized in 500 ...
... periods . Incubations were performed in a shaking water bath at 37 ° C . After the pulse period , the medium was withdrawn and the islets were washed twice with buffer . Islets from half of the incubation vials were homogenized in 500 ...
Pagina 100
... period . After the 120 min . chase period ( 3.0 mg / ml glucose ) some radioactivity was still recovered in the islets ( 55 ± 3 % in proinsulin ) while significant levels were now found in the media ( 50 ± 3 % of the radioactivity was ...
... period . After the 120 min . chase period ( 3.0 mg / ml glucose ) some radioactivity was still recovered in the islets ( 55 ± 3 % in proinsulin ) while significant levels were now found in the media ( 50 ± 3 % of the radioactivity was ...
Sommario
Methodology | 1 |
Applications to Physiology | 72 |
Tokuko Saito and Kazuo Shizume | 86 |
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ACTH activity albumin amylase anti antibody antigen antisera antiserum arginine assay basal binding biological blood glucose bovine buffer C-peptide chymotrypsin chymotrypsinogen clin concentration cross-reactivity cyclic AMP diabetes dilution dose effect eluted Endocr Endocrinology enzymes erythropoietin estradiol estrogen extract fraction Freychet gastrin glucagon glucose tolerance GnRH gonadotropin granulated growth hormone HTSH human immunoassay immunoreactive increase incubation injection insulin levels insulin response islets isolated liver cells labelled LH and FSH LH response LH-RH LHRH luteinizing hormone measured membranes method mg/ml minutes ng/ml normal obesity oral pancreas pancreatic patients peak perifusion pituitary plasma insulin porcine pork preparations progesterone proinsulin proinsulin and insulin protein puberty purified rabbit radioactivity radioimmunoassay receptors releasing hormone S.A. Berson samples Sephadex serum sham feeding specific standard curve steroid stimulation subjects subunit Table theophylline thyroxine tion trypsin unlabeled