Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. BersonRolf Luft, Solomon A. Berson, Rosalyn Sussman Yalow, J. P. Accary Thieme, 1974 - 195 pagine |
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Pagina 99
... radioactivity assayed in successive 1.0 ml fractions . Results When granulated islets were incubated for 120 min . with 3H - leucine and 3.0 mg / ml glucose , radioactivity was recovered in three maior G - 50 peaks ( Figure 1 ) . The ...
... radioactivity assayed in successive 1.0 ml fractions . Results When granulated islets were incubated for 120 min . with 3H - leucine and 3.0 mg / ml glucose , radioactivity was recovered in three maior G - 50 peaks ( Figure 1 ) . The ...
Pagina 100
... radioactivity in proinsulin ( Fig . 4 ) . No significant release of radioactive proinsulin or insulin was detected in the media during this 60 min . pulse period . After the 120 min . chase period ( 3.0 mg / ml glucose ) some radioactivity ...
... radioactivity in proinsulin ( Fig . 4 ) . No significant release of radioactive proinsulin or insulin was detected in the media during this 60 min . pulse period . After the 120 min . chase period ( 3.0 mg / ml glucose ) some radioactivity ...
Pagina 101
... radioactivity patterns for the pulse islet and media samples are exhibited on the left while those for the chase samples on the right . These G - 50 radioactivity pattern are representa- tive of 5 consecutive experiments . The mean ...
... radioactivity patterns for the pulse islet and media samples are exhibited on the left while those for the chase samples on the right . These G - 50 radioactivity pattern are representa- tive of 5 consecutive experiments . The mean ...
Sommario
Methodology | 1 |
Applications to Physiology | 72 |
Tokuko Saito and Kazuo Shizume | 86 |
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ACTH activity albumin amylase anti antibody antigen antisera antiserum arginine assay basal binding biological blood glucose bovine buffer C-peptide chymotrypsin chymotrypsinogen clin concentration cross-reactivity cyclic AMP diabetes dilution dose effect eluted Endocr Endocrinology enzymes erythropoietin estradiol estrogen extract fraction Freychet gastrin glucagon glucose tolerance GnRH gonadotropin granulated growth hormone HTSH human immunoassay immunoreactive increase incubation injection insulin levels insulin response islets isolated liver cells labelled LH and FSH LH response LH-RH LHRH luteinizing hormone measured membranes method mg/ml minutes ng/ml normal obesity oral pancreas pancreatic patients peak perifusion pituitary plasma insulin porcine pork preparations progesterone proinsulin proinsulin and insulin protein puberty purified rabbit radioactivity radioimmunoassay receptors releasing hormone S.A. Berson samples Sephadex serum sham feeding specific standard curve steroid stimulation subjects subunit Table theophylline thyroxine tion trypsin unlabeled